This study delves into the polarization properties of various hair colors using several techniques, including polarization ray tracing, full Stokes, and Mueller matrix imaging. Our analysis involved studying hair in both indoor and outdoor settings under varying lighting conditions. Our results demonstrate a strong correlation between hair color and the degree of linear polarization. Specifically, light-colored hair, such as white and blond, exhibits high albedo and low DoLP. In contrast, dark hair, like black and brown hair, has low albedo and high DoLP. Our research also revealed that a single hair strand displays high diattenuation near specular reflections but high depolarization in areas with diffuse reflections. Additionally, we investigated the wavelength dependency of the polarization properties by comparing the Mueller matrix under illumination at 450 nm and 589 nm. Our investigation demonstrates the impact of hair shade and color on polarization properties and the Umov effect.
Stokes polarimeter based endoscopes are emerging as an area of technology where polarization imaging can greatly impact clinical care by improving diagnostic tools without the use of exogenous contrast. Image acquisition in minimally invasive surgical settings is often beset by inherently limited illumination. A comprehensive analysis of how signal-to-noise (SNR) propagates through Stokes polarimetric outcomes such as degree of linear polarization (DoLP) and angle of polarization (AoP) in low light is important for future interpretation of data acquired in low-light conditions. A previously developed theoretical model of quantitative polarized light imaging (QPLI) analysis described SNR as a function of both incident light intensity and DoLP. When polarized light interacts with biological tissues, the resultant DoLP of exiting light is dependent on the underlying tissue microstructure. Therefore, in this study we explore how low light impacts SNR of QPLI outcomes of DoLP and AoP differently in tissue phantoms of varying microstructures. Data are compared to theoretical solutions of SNR of DoLP and AoP. Tissues were additionally loaded to varying magnitudes of strain to investigate how variable SNR affects the ability to discern dynamic realignment in biological tissues. We observed a high degree of congruency between experimental and theoretical data, with SNR depending on both light intensity and DoLP. Additionally, we found that AoP may have a greater resilience to noise overall than DoLP and, as such, may be particularly useful in conditions where light is inherently limited.
more » « less- NSF-PAR ID:
- 10498792
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Biomedical Optics Express
- Volume:
- 15
- Issue:
- 5
- ISSN:
- 2156-7085
- Format(s):
- Medium: X Size: Article No. 2798
- Size(s):
- Article No. 2798
- Sponsoring Org:
- National Science Foundation
More Like this
-
Abstract -
Moonlight has a partial polarization state that varies with the lunar phase angle. This needs to be characterized for the Moon to be used as a calibration source for polarization-sensitive instruments or as the illumination source in nighttime passive remote sensing. To contribute to this characterization, a pixelated polarization imager was calibrated with a telescope and with a telephoto lens and used to record images of the Moon at 40 phase angles from -138° to 125°. High-resolution images of relative intensity, degree of linear polarization (DoLP), and angle of polarization (AoP) were recorded in a spectral band from 400-1000 nm and were used to calculate disk-averaged DoLP values. These images are made available to readers. Results showed higher DoLP in regions of lower reflectivity (the Umov effect), higher DoLP for waning phases (maximum DoLP of 8.3%) than for waxing (maximum DoLP of 6.8%), and consistent DoLP to disk-averaged values published previously for wavelengths contained within our broader band.
-
Recent advancements in nanofabrication technology has led to commercialization of single-chip polarization and color-polarization imaging sensors in the visible spectrum. Novel applications have arisen with the emergence of these sensors leading to questions about noise in the reconstructed polarization images. In this paper, we provide theoretical analysis for the input and output referred noise for the angle and degree of linear polarization information. We validated our theoretical model with experimental data collected from a division of focal plane polarization sensor. Our data indicates that the noise in the angle of polarization images depends on both incident light intensity and degree of linear polarization and is independent of the incident angle of polarization. However, noise in degree of linear polarization images depends on all three parameters: incident light intensity, angle and degree of linear polarization. This theoretical model can help guide the development of imaging setups to record optimal polarization information.
-
Kidney cancer is a kind of high mortality cancer because of the difficulty in early diagnosis and the high metastatic dissemination in treatments. The surgical resection of tumors is the most effective treatment for renal cancer patients. However, precise assessment of tumor margins is a challenge during surgical resection. The objective of this study is to demonstrate an optical imaging tool in precisely distinguishing kidney tumor borders and identifying tumor zones from normal tissues to assist surgeons in accurately resecting tumors from kidneys during the surgery. 30 samples from six human kidneys were imaged using polarization-sensitive optical coherence tomography (PS-OCT). Cross-sectional, enface, and spatial information of kidney samples were obtained for microenvironment reconstruction. Polarization parameters (phase retardation, optic axis direction, and degree of polarization uniformity (DOPU) and Stokes parameters (Q, U, and V) were utilized for multiparameter analysis. To verify the detection accuracy of PS-OCT, H&E histology staining and dice-coefficient were utilized to quantify the performance of PS-OCT in identifying tumor borders and regions. In this study, tumor borders were clearly identified by PS-OCT imaging, which outperformed the conventional intensity-based OCT. With H&E histological staining as golden standard, PS-OCT precisely identified the tumor regions and tissue distributions at different locations and different depths based on polarization and Stokes parameters. Compared to the traditional attenuation coefficient quantification method, PS-OCT demonstrated enhanced contrast of tissue characteristics between normal and cancerous tissues due to the birefringence effects. Our results demonstrated that PS-OCT was promising to provide imaging guidance for the surgical resection of kidney tumors and had the potential to be used for other human kidney surgeries in clinics such as renal biopsy.more » « less
-
Polarimeters have broad applications in remote sensing, astronomy, and biomedical imaging to measure the emitted, reflected, or transmitted state of polarization. An intrinsic coincident (IC) full-Stokes polarimeter was previously demonstrated by our group, in a free space configuration, by using stain-aligned polymer-based organic photovoltaics. To minimize the model’s complexity, these were tilted to avoid crosstalk from back-reflections. We present a theoretical model of a monolithic IC polarimeter that considers the back-reflection’s influence for on-axis light. The model was validated using a monolithic four-detector polarimeter, which achieved an error of less than 3%. Additionally, an off-axis model was produced and validated for a simpler two detector polarimeter, demonstrating an error between the TM and TE polarized components of less than 3% for angles spanning an 18° incidence cone.