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Title: Endoplasmic Reticulum Calcium Mediates Drosophila Wing Development
The temporal dynamics of morphogen presentation impacts transcriptional responses and tissue patterning (1). However, the mechanisms controlling morphogen release are far from clear. We found that inwardly rectifying potassium (Irk) channels regulate endogenous transient increases in intracellular calcium and Bone Morphogenetic Protein (BMP/Dpp) release for Drosophila wing development (2). Inhibition of Irk channels reduces BMP/Dpp signaling, and ultimately disrupts wing morphology (2, 3). Ion channels impact development of several tissues and organisms in which BMP signaling is essential (2-15). In neurons and pancreatic beta cells, Irk channels modulate membrane potential to affect intracellular Ca++ to control secretion of neurotransmitters and insulin (15-21). Based on Irk activity in neurons, we hypothesized that electrical activity controls endoplasmic reticulum Ca++ release into the cytoplasm to regulate the release of BMP. To test this hypothesis, we reduced expression of proteins that control endoplasmic reticulum calcium (Stim, Orai, SERCA, SK, and Best2) and documented wing phenotypes. We found that reduced Stim and SERCA function decreases amplitude and frequency of endogenous calcium transients in the wing disc and reduced Dpp/BMP release in the wing disc. Together, our results suggest control of endoplasmic reticulum is required for Dpp/BMP release.  more » « less
Award ID(s):
1945916
PAR ID:
10499556
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Mary Ann Liebert
Date Published:
Journal Name:
Bioelectricity
Volume:
5
Issue:
4
ISSN:
2576-3105
Page Range / eLocation ID:
290 to 306
Subject(s) / Keyword(s):
calcium, transients, endoplasmic reticulum, SERCA, Stim, Orai, bestrophin, Drosophila wing development, wing venation, BMP, bone morphogenic protein
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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