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Title: Non-Native Site-Selective Enzyme Catalysis
The ability to site-selectively modify equivalent functional groups in a molecule has the potential to streamline syntheses and increase product yields by lowering step counts. Enzymes catalyze site-selective transformations throughout primary and secondary metabolism, but leveraging this capability for non-native substrates and reactions requires a detailed understanding of the potential and limitations of enzyme catalysis and how these bounds can be extended by protein engineering. In this review, we discuss representative examples of site-selective enzyme catalysis involving functional group manipulation and C–H bond functionalization. We include illustrative examples of native catalysis, but our focus is on cases involving non-native substrates and reactions often using engineered enzymes. We then discuss the use of these enzymes for chemoenzymatic transformations and target-oriented synthesis and conclude with a survey of tools and techniques that could expand the scope of non-native site-selective enzyme catalysis.  more » « less
Award ID(s):
2154726
PAR ID:
10504466
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
ACS
Date Published:
Journal Name:
Chemical Reviews
Volume:
123
Issue:
16
ISSN:
0009-2665
Page Range / eLocation ID:
10381 to 10431
Subject(s) / Keyword(s):
Site-selective catalysis biocatalysis chemoenzymatic C-H functionalization
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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