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Title: Moment-based metrics for molecules computable from cryogenic electron microscopy images
Abstract Single-particle cryogenic electron microscopy (cryo-EM) is an imaging technique capable of recovering the high-resolution three-dimensional (3D) structure of biological macromolecules from many noisy and randomly oriented projection images. One notable approach to 3D reconstruction, known as Kam’s method, relies on the moments of the two-dimensional (2D) images. Inspired by Kam’s method, we introduce a rotationally invariant metric between two molecular structures, which does not require 3D alignment. Further, we introduce a metric between a stack of projection images and a molecular structure, which is invariant to rotations and reflections and does not require performing 3D reconstruction. Additionally, the latter metric does not assume a uniform distribution of viewing angles. We demonstrate the uses of the new metrics on synthetic and experimental datasets, highlighting their ability to measure structural similarity.  more » « less
Award ID(s):
2309782 2009753
PAR ID:
10510076
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Cambridge University Press
Date Published:
Journal Name:
Biological Imaging
Volume:
4
ISSN:
2633-903X
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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