An official website of the United States government
Abstract BackgroundUrbanization can influence disease vectors by altering larval habitat, microclimates, and host abundance. The global increase in urbanization, especially in Africa, is likely to alter vector abundance and pathogen transmission. We investigated the effect of urbanization and weather on the abundance of two mosquitoes,Aedes aegyptiandAedes albopictus, and infection with dengue, chikungunya, and Zika viruses at 63 sites in six cities spanning a 900-km latitudinal range in Cameroon, Central Africa. MethodsWe used human landing catches and backpack-mounted aspirators to sample mosquitoes and collected larval habitat, host availability, and weather (temperature, precipitation, humidity) data for each site in each city. We analyzed land use and land cover information and satellite photos at varying radii around sites (100 m to 2 km) to quantify the extent of urbanization and the number of structures around each site. We used a continuous urbanization index (UI; range 0–100) that increased with impermeable surface and decreased with forest cover. ResultsUrbanization increased larval habitat, human host availability, andAe. aegyptimosquito abundance.Aedes aegyptiabundance increased 1.7% (95% CI 0.69–2.7%) with each 1 unit increase in the urbanization index in all six cities (Douala, Kribi, Yaounde, Ngaoundere, Garoua, and Maroua) with a 5.4-fold increase from UI = 0 to UI = 100, and also increased with rainfall. In contrast,Ae. albopictusabundance increased with urbanization in one city, but showed no influence of urbanization in two other cites. Across three cities,Ae. albopictusabundance increased with rainfall, temperature, and humidity. Finally, we did not detect Zika, dengue, or chikungunya viruses in any specimens, and found weak evidence of interspecific competition in analyses of adult population growth rates. ConclusionsThese results show that urbanization consistently increasesAe. aegyptiabundance across a broad range of habitats in Central Africa, while effects onAe. albopictuswere more variable and the abundance of both species were influenced by rainfall. Future urbanization of Africa will likely increaseAe. aegyptiabundance, and climate change will likely alter abundance of both species through changes in precipitation and temperature. Graphical Abstract
more »
« less
Behavioral interplay between mosquito and mycolactone produced by Mycobacterium ulcerans and bacterial gene expression induced by mosquito proximity
Mycolactone is a cytotoxic lipid metabolite produced byMycobacterium ulcerans, the environmental pathogen responsible for Buruli ulcer, a neglected tropical disease.Mycobacterium ulceransis prevalent in West Africa, particularly found in lentic environments, where mosquitoes also occur. Researchers hypothesize mosquitoes could serve as a transmission mechanism resulting in infection byM.ulceranswhen mosquitoes pierce skin contaminated withM.ulcerans. The interplay between the pathogen, mycolactone, and mosquito is only just beginning to be explored. A triple-choice assay was conducted to determine the host-seeking preference ofAedes aegyptibetweenM.ulceranswildtype (MU, mycolactone active) and mutant (MUlac-, mycolactone inactive). Both qualitative and quantitative differences in volatile organic compounds’ (VOCs) profiles of MU and MUlac-were determined by GC-MS. Additionally, we evaluated the interplay betweenAe.aegyptiproximity andM.ulceransmRNA expression. The results showed that mosquito attraction was significantly greater (126.0%) to an artificial host treated with MU than MUlac-. We found that MU and MUlacproduced differential profiles of VOCs associated with a wide range of biological importance from quorum sensing (QS) to human odor components. RT-qPCR assays showed that mycolactone upregulation was 24-fold greater for MU exposed toAe.aegyptiin direct proximity. Transcriptome data indicated significant induction of ten chromosomal genes of MU involved in stress responses and membrane protein, compared to MUlac-when directly having access to or in near mosquito proximity. Our study provides evidence of possible interkingdom interactions between unicellular and multicellular species that MU present on human skin is capable of interreacting with unrelated species (i.e., mosquitoes), altering its gene expression when mosquitoes are in direct contact or proximity, potentially impacting the production of its VOCs, and consequently leading to the stronger attraction of mosquitoes toward human hosts. This study elucidates interkingdom interactions between viableM.ulceransbacteria andAe.aegyptimosquitoes, which rarely have been explored in the past. Our finding opens new doors for future research in terms of disease ecology, prevalence, and pathogen dispersal outside of theM.ulceranssystem.
more »
« less
- Award ID(s):
- 1911457
- PAR ID:
- 10510611
- Editor(s):
- de_Oliveira, Mozaniel Santana
- Publisher / Repository:
- National Library of Science
- Date Published:
- Journal Name:
- PLOS ONE
- Volume:
- 18
- Issue:
- 8
- ISSN:
- 1932-6203
- Page Range / eLocation ID:
- e0289768
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
Abstract Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and identify microbial taxa and assemblages associated with health or disease. However, no such approaches have been developed to manipulate the microbiota of mosquitoes. Results Here, we developed an approach to transfer entire microbial communities between mosquito cohorts. We undertook transfers between ( Culex quinquefasciatus to Aedes aegypti ) and within ( Ae. aegypti to Ae. aegypti ) species to validate the approach and determine the number of mosquitoes required to prepare donor microbiota. After the transfer, we monitored mosquito development and microbiota dynamics throughout the life cycle. Typical holometabolous lifestyle-related microbiota structures were observed, with higher dynamics of microbial structures in larval stages, including the larval water, and less diversity in adults. Microbiota diversity in recipient adults was also more similar to the microbiota diversity in donor adults. Conclusions This study provides the first evidence for successful microbiome transplantation in mosquitoes. Our results highlight the value of such methods for studying mosquito-microbe interactions and lay the foundation for future studies to elucidate the factors underlying microbiota acquisition, assembly, and function in mosquitoes under controlled conditions.more » « less
-
Mosquitoes are prolific vectors of human pathogens, therefore a clear and accurate understanding of the organization of their antimicrobial defenses is crucial for informing the development of transmission control strategies. The canonical infection response in insects, as described in the insect modelDrosophila melanogaster, is pathogen type-dependent, with distinct stereotypical responses to Gram-negative bacteria and Gram-positive bacteria/fungi mediated by the activation of the Imd and Toll pathways, respectively. To determine whether this pathogen-specific discrimination is shared by mosquitoes, we used RNAseq to capture the genome-wide transcriptional response ofAedes aegyptiandAnopheles gambiae(s.l.) to systemic infection with Gram-negative bacteria, Gram-positive bacteria, yeasts, and filamentous fungi, as well as challenge with heat-killed Gram-negative, Gram-positive, and fungal pathogens. From the resulting data, we found thatAe. aegyptiandAn. gambiaeboth mount a core response to all categories of infection, and this response is highly conserved between the two species with respect to both function and orthology. When we compared the transcriptomes of mosquitoes infected with different types of bacteria, we observed that the intensity of the transcriptional response was correlated with both the virulence and growth rate of the infecting pathogen. Exhaustive comparisons of the transcriptomes of Gram-negative-challenged versus Gram-positive-challenged mosquitoes yielded no difference in either species. InAe. aegypti, however, we identified transcriptional signatures specific to bacterial infection and to fungal infection. The bacterial infection response was dominated by the expression of defensins and cecropins, while the fungal infection response included the disproportionate upregulation of an uncharacterized family of glycine-rich proteins. These signatures were also observed inAe. aegyptichallenged with heat-killed bacteria and fungi, indicating that this species can discriminate between molecular patterns that are specific to bacteria and to fungi.more » « less
-
ABSTRACT Climate warming is expected to substantially impact the global landscape of mosquito‐borne disease, but these impacts will vary across disease systems and regions. Understanding which diseases, and where within their distributions, these impacts are most likely to occur is critical for preparing public health interventions. While research has centered on potential warming‐driven expansions in vector transmission, less is known about the potential for vectors to experience warming‐driven stress or even local extirpations. In conservation biology, species risk from climate warming is often quantified through vulnerability indices such as thermal safety margins—the difference between an organism's upper thermal limit and its habitat temperature. Here, we estimated thermal safety margins for 8 mosquito species that are the vectors of malaria, dengue, chikungunya, Zika, West Nile and other major arboviruses, across their known ranges to investigate which mosquitoes and regions are most and least vulnerable to climate warming. We find that several of the most medically important mosquito vector species, includingAe. aegyptiandAn. gambiae, have positive thermal safety margins across the majority of their ranges when realistic assumptions of mosquito behavioral thermoregulation are incorporated. On average, the lowest climate vulnerability, in terms of both the magnitude and duration of thermal safety, was just south of the equator and at northern temperate range edges, and the highest climate vulnerability was in the subtropics. Mosquitoes living in regions including the Middle East, the western Sahara, and southeastern Australia, which are largely comprised of desert and xeric shrubland biomes, have the highest climate vulnerability across vector species.more » « less
-
The markedly anthropophilic and endophilic behaviors of Aedes aegypti (L.) make it a very efficient vector of dengue, chikungunya, and Zika viruses. Although a large body of research has investigated the immature habitats and conditions for adult emergence, relatively few studies have focused on the indoor resting behavior and distribution of vectors within houses. We investigated the resting behavior of Ae. aegypti indoors in 979 houses of the city of Acapulco, Mexico, by performing exhaustive indoor mosquito collections to describe the rooms and height at which mosquitoes were found resting. In total, 1,403 adult and 747 female Ae. aegypti were collected, primarily indoors (98% adults and 99% females). Primary resting locations included bedrooms (44%), living rooms (25%), and bathrooms (20%), followed by kitchens (9%). Aedes aegypti significantly rested below 1.5 m of height (82% adults, 83% females, and 87% bloodfed females); the odds of finding adult Ae. aegypti mosquitoes below 1.5 m was 17 times higher than above 1.5 m. Our findings provide relevant information for the design of insecticide-based interventions selectively targeting the adult resting population, such as indoor residual spraying.more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript
- Journal Article:
- https://doi.org/10.1371/journal.pone.0289768
