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Abstract Understanding host immune function and ecoimmunology is increasingly important at a time when emerging infectious diseases (EIDs) threaten wildlife. One EID that has emerged and spread widely in recent years is chytridiomycosis, caused by the fungal pathogenBatrachochytrium dendrobatidis(Bd), which is implicated unprecedented amphibian declines around the world. The impacts ofBdhave been severe for many amphibian species, but some populations have exhibited signs of persistence, and even recovery, in some regions. Many mechanisms may underpin this pattern and amphibian immune responses are likely one key component. Although we have made great strides in understanding amphibian immunity, the complement system remains poorly understood. The complement system is a nonspecific, innate immune defense that is known to enhance other immune responses. Complement activation can occur by three different biochemical pathways and result in protective mechanisms, such as inflammation, opsonization, and pathogen lysis, thereby providing protection to the host. We currently lack an understanding of complement pathway activation for chytridiomycosis, but several studies have suggested that it may be a key part of an early and robust immune response that confers host resistance. Here, we review the available research on the complement system in general as well as amphibian complement responses toBdinfection. Additionally, we propose future research directions that will increase our understanding of the amphibian complement system and other immune responses toBd. Finally, we suggest how a deeper understanding of amphibian immunity could enhance the conservation and management of amphibian species that are threatened by chytridiomycosis.
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The complement system and its involvement in inhibition of Batrachochytriym dendrobatidis, a lethal fungal pathogen of amphibians
The field of ecological immunology, or ecoimmunology, has provided valuable insights on the immune responses of diverse host organisms threatened by infectious diseases in many different environments. One infectious disease that has been particularly notable for its impacts on host populations is amphibian chytridiomycosis, which has been linked with amphibian declines around the world. Amphibian immune responses to the pathogen that causes chytridiomycosis (Batrachochytriym dendrobatidis) are not well understood but thought to involve innate immune factors, including the complement system. In this study, we tested the ability of complement proteins to inhibitB. dendrobatidisinin vitrochallenge assays. We found that complement proteins from amphibian plasma that were not heat inactivated reduced the viability and growth ofB. dendrobatidis.The inhibitory efficacy was similar to effects onPseudomonas fluorescens, a bacterium that is known to be inhibited by complement protein activation. These findings suggest inhibition ofB. dendrobatidisthat is consistent with the involvement of the complement system. In addition, we provide methods for standardizing pathogen killing assays, and set a foundation for further investigations on the amphibian complement system and other immune responses to amphibian chytridiomycosis.
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- PAR ID:
- 10511236
- Publisher / Repository:
- Frontiers in Amphibian and Reptile Science
- Date Published:
- Journal Name:
- Frontiers in Amphibian and Reptile Science
- Volume:
- 2
- ISSN:
- 2813-6780
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.3389/famrs.2024.1294491
