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Title: Low‐cost, 3D printed irradiation system for in vitro photodynamic therapy experiments
Abstract The development of a suitable irradiation setup is essential for in vitro experiments in photodynamic therapy (PDT). While various irradiation systems have been developed for PDT, only a few offer practical and high‐quality setups for precise and reproducible results in cell culture experiments. This report introduces a cost‐effective illumination setup designed for in vitro photodynamic treatments. The setup consists of a commercially available light‐emitting diode (LED) lamp, a cooling unit, and a specially designed 3D‐printed enclosure to accommodate a multiwell plate insert. The LED lamp is versatile, supporting various irradiation wavelengths and adjustable illumination fields, ensuring consistent and reliable performance. The study evaluates the setup through various parameters, including photon flux density, illumination uniformity, photon distribution across the multiwell plate, and temperature changes during irradiation. In addition, the effectiveness of the LED‐based illumination system is tested by treating mouse mammary breast carcinoma cells (4T1) with Rose Bengal and LED irradiation at around 525 nm. The resulting IC50of 5.2 ± 0.9 μM and a minimum media temperature change of ca. 1.2°C indicate a highly promising LED‐based setup that offers a cost‐effective and technically feasible solution for achieving consistent, reproducible, and uniform irradiation, enhancing research capabilities and potential applications.  more » « less
Award ID(s):
2246805
PAR ID:
10514683
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Photochemistry and Photobiology
Volume:
100
Issue:
3
ISSN:
0031-8655
Page Range / eLocation ID:
530 to 540
Subject(s) / Keyword(s):
Photodynamic Therapy in vitro irradiation setup
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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