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Title: Development of an efficient, effective, and economical technology for proteome analysis
We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome.  more » « less
Award ID(s):
1817651 2244127
PAR ID:
10526789
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ; ;
Publisher / Repository:
Cell Press
Date Published:
Journal Name:
Cell Reports Methods
Volume:
4
Issue:
6
ISSN:
2667-2375
Page Range / eLocation ID:
100796
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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