Title: Genome-sequenced bacterial collection from sorghum aerial root mucilage
ABSTRACT A collection of 47 bacteria isolated from the mucilage of aerial roots of energy sorghum is available at the Great Lakes Bioenergy Research Center, Michigan State University, Michigan, USA. We enriched bacteria with putative plant-beneficial phenotypes and included information on phenotypic diversity, taxonomy, and whole genome sequences. more »« less
ABSTRACT A collection of 44 isolates isolated from the epicuticular wax of stems of energy sorghum is available at the Great Lakes Bioenergy Researcher Center, Michigan State University, MI, USA. We enriched bacteria with putative plant-beneficial phenotypes and include information on their phenotypic diversity, taxonomy, and whole-genome sequences.
Figueroa, Héctor Fox; Grady, CJ; Cortez, Maria_Beatriz_de Souza; Beach, Jim; Stewart, Aimee; Soltis, Douglas E; Soltis, Pamela S; Smith, Stephen A
(, Research Square)
Abstract Distribution models are widely used to understand landscape biodiversity patterns, facilitate evolutionary and ecological studies, and for making informed conservation decisions. While it is common to examine consequences of climate change, impacts of land use on distributions, a major factor in limiting ranges and corridors between populations, are less well understood. Here, we use distribution models to quantify changes in biodiversity due to land use for Michigan floral assemblages. We leveraged a distribution model dataset (1930 species) integrated with dated phylogenetic information and USGS land use maps to parse Michigan areas with unsuitable habitat. Additionally, we quantify the degree of high-quality habitat lost for each species, identifying those most strongly impacted by land use changes. Approx. 39% of Michigan terrestrial habitat fell within “unsuitable” land use categories. Sites predicted to harbor the most species based on climatic variables were those sites that lost the greatest proportion due to land use changes. Further, excluded sites were preferentially those composed of more phylogenetically even communities. Overall, the impact of land use changes on community species richness was the preferential loss of sites with the predicted highest biodiversity. For phylodiversity metrics, land use changes increased the degree of community phylogenetic clustering. This results in overall decreased phylodiversity, leading to assemblages less equipped to respond to rapid climatic changes. Our results confirm land use to be a major, but somewhat overlooked, factor impacting local diversity dynamics and illustrate how local-scale land use impacts regional-scale richness and phylodiversity patterns, likely leading to increased community fragility.
Abstract BackgroundDuring the bloom season, the colonial cyanobacteriumMicrocystisforms complex aggregates which include a diverse microbiome within an exopolymer matrix. Early research postulated a simple mutualism existing with bacteria benefitting from the rich source of fixed carbon andMicrocystisreceiving recycled nutrients. Researchers have since hypothesized thatMicrocystisaggregates represent a community of synergistic and interacting species, an interactome, each with unique metabolic capabilities that are critical to the growth, maintenance, and demise ofMicrocystisblooms. Research has also shown that aggregate-associated bacteria are taxonomically different from free-living bacteria in the surrounding water. Moreover, research has identified little overlap in functional potential betweenMicrocystisand members of its microbiome, further supporting the interactome concept. However, we still lack verification of general interaction and know little about the taxa and metabolic pathways supporting nutrient and metabolite cycling withinMicrocystisaggregates. ResultsDuring a 7-month study of bacterial communities comparing free-living and aggregate-associated bacteria in Lake Taihu, China, we found that aerobic anoxygenic phototrophic (AAP) bacteria were significantly more abundant withinMicrocystisaggregates than in free-living samples, suggesting a possible functional role for AAP bacteria in overall aggregate community function. We then analyzed gene composition in 102 high-quality metagenome-assembled genomes (MAGs) of bloom-microbiome bacteria from 10 lakes spanning four continents, compared with 12 completeMicrocystisgenomes which revealed that microbiome bacteria andMicrocystispossessed complementary biochemical pathways that could serve in C, N, S, and P cycling. Mapping published transcripts fromMicrocystisblooms onto a comprehensive AAP and non-AAP bacteria MAG database (226 MAGs) indicated that observed high levels of expression of genes involved in nutrient cycling pathways were in AAP bacteria. ConclusionsOur results provide strong corroboration of the hypothesizedMicrocystisinteractome and the first evidence that AAP bacteria may play an important role in nutrient cycling withinMicrocystisaggregate microbiomes.
D'Antuono, Carmela; Anderson, Kayla; Afuso, Joseph; Huang, Michelle; Robichaud, Jennifer; Rund, Samuel
(, Biodiversity Data Journal)
Approximately twenty-one years of historical mosquito abundance and species surveillance data, collected by the University of Notre Dame and the St. Joseph County (IN) Health Department, from 1976 to 1997 are made available following a data rescue effort. St. Joseph County is a county in Indiana, located on the Michigan-Indiana border, 35 miles from Lake Michigan. The collected data will allow for trends in species to be followed over a wide time range and facilitate further research regarding mosquito-borne diseases, species distribution, phenology and ecological changes over time.
Folvarska, Veronika; Thomson, San Marie; Lu, Zihao; Adelgren, Maya; Schmidt, Adam; Newton, Ryan J; Wang, Yin; McNamara, Patrick J
(, Environmental Science: Advances)
Antibiotic resistance is a public health crisis. Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are present in drinking water distribution systems. Metals are known selective pressures for antibiotic resistance, and metallic corrosion products are found within drinking water distribution systems due to the corrosion of metal pipes. While corrosion products are a source of metals, the impact of specific corrosion products on antibiotic resistance has not been investigated. The objective of this study was to determine the impact of six corrosion products—CuO, Cu2O, Pb5(PO4)3OH, b-PbO2, Fe3O4, and a-FeOOH—on the abundance of ARB and ARGs. Lab-scale microcosms were seeded with source water from Lake Michigan and amended with individual corrosion products. In general, copper and lead corrosion products increased antibiotic resistance, although not universally across different ARB and ARG types. Concentration and speciation of copper and lead corrosion products were found to have an impact on antibiotic resistance profiles. Meanwhile, iron corrosion products had minimal impact on antibiotic resistance. Overall, this study sheds light on how pipe materials may impact antibiotic resistance as a result of corrosion products.
Mechan-Llontop, Marco E, Mullet, John, and Shade, Ashley. Genome-sequenced bacterial collection from sorghum aerial root mucilage. Retrieved from https://par.nsf.gov/biblio/10542070. Microbiology Resource Announcements 12.12 Web. doi:10.1128/MRA.00468-23.
Mechan-Llontop, Marco E, Mullet, John, and Shade, Ashley.
"Genome-sequenced bacterial collection from sorghum aerial root mucilage". Microbiology Resource Announcements 12 (12). Country unknown/Code not available: American Society for Microbiology. https://doi.org/10.1128/MRA.00468-23.https://par.nsf.gov/biblio/10542070.
@article{osti_10542070,
place = {Country unknown/Code not available},
title = {Genome-sequenced bacterial collection from sorghum aerial root mucilage},
url = {https://par.nsf.gov/biblio/10542070},
DOI = {10.1128/MRA.00468-23},
abstractNote = {ABSTRACT A collection of 47 bacteria isolated from the mucilage of aerial roots of energy sorghum is available at the Great Lakes Bioenergy Research Center, Michigan State University, Michigan, USA. We enriched bacteria with putative plant-beneficial phenotypes and included information on phenotypic diversity, taxonomy, and whole genome sequences.},
journal = {Microbiology Resource Announcements},
volume = {12},
number = {12},
publisher = {American Society for Microbiology},
author = {Mechan-Llontop, Marco E and Mullet, John and Shade, Ashley},
editor = {Baltrus, David A}
}
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