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Title: Synthetic Genes For Dynamic Regulation Of DNA‐Based Receptors
Abstract We present a strategy to control dynamically the loading and release of molecular ligands from synthetic nucleic acid receptors using in vitro transcription. We demonstrate this by engineering three model synthetic DNA‐based receptors: a triplex‐forming DNA complex, an ATP‐binding aptamer, and a hairpin strand, whose ability to bind their specific ligands can be cotranscriptionally regulated (activated or inhibited) through specific RNA molecules produced by rationally designed synthetic genes. The kinetics of our DNA sensors and their genetically generated inputs can be captured using differential equation models, corroborating the predictability of the approach used. This approach shows that highly programmable nucleic acid receptors can be controlled with molecular instructions provided by dynamic transcriptional systems, illustrating their promise in the context of coupling DNA nanotechnology with biological signaling.  more » « less
Award ID(s):
2107483
PAR ID:
10545540
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Angewandte Chemie
Volume:
136
Issue:
17
ISSN:
0044-8249
Subject(s) / Keyword(s):
DNA nanotechnology, gene networks, receptors, modeling
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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