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Title: Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts
Herein we report the assessment of the effects of shockwave (SW) impacts on adult rat hippocampal progenitor cell (AHPC) neurospheres (NSs), which are used as in vitro brain models, for enhancing our understanding of the mechanisms of traumatic brain injury (TBI). The assessment has been achieved by using culture dishes and a new microchip. The microchip allows the chemicals released from the brain models cultured inside the cell culture chamber under SW impacts to diffuse to the nano sensors in adjacent sensor chambers through built-in diffusion barriers, which are used to prevent the cells from entering the sensor chambers, thereby mitigating the biofouling issues of the sensor surface. Experiments showed the negative impact of the SW on the viability, proliferation, and differentiation of the cells within the NSs. A qPCR gene expression analysis was performed and appeared to confirm some of the immunocytochemistry (ICC) results. Finally, we demonstrated that the microchip can be used to monitor lactate dehydrogenase (LDH) released from the AHPC-NSs subjected to SW impacts. As expected, LDH levels changed when AHPC-NSs were injured by SW impacts, verifying this chip can be used for assessing the degrees of injuries of AHPC-NSs by monitoring LDH levels. Taken together, these results suggest the feasibility of using the chip to better understand the interactions between SW impacts and in vitro brain models, paving a way for potentially establishing in vitro TBI models on a chip.  more » « less
Award ID(s):
2024797
PAR ID:
10548328
Author(s) / Creator(s):
; ; ; ; ;
Publisher / Repository:
ACS Publications
Date Published:
Journal Name:
ACS Applied Materials & Interfaces
Volume:
16
Issue:
26
ISSN:
1944-8244
Page Range / eLocation ID:
33246 to 33258
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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