Diel metatranscriptomes capture cyanobacteria-dominated Lake Erie community response to episodic events American Society for Microbiology 11/12/2024 10555044 10.1128/mra.00659-24 Microbiology Resource Announcements 2576-098X 13 11 Brittany N ZepernickAnna G BoegeholdE Anders KiledalEmily E ChaseLauren N HartKatelyn A HoughtonRobbie M MartinPaige A WilliamsEmma C JohnsonParis K SchofieldRose M CorySubba Rao ChagantiCasey M GodwinTrisha L SpanbauerGregory J DickReagan M ErreraSteven W WilhelmKenneth M Stedman ABSTRACT <p>Here, we report on the raw and coassembled metatranscriptomes of 39 Lake Erie surface (1.0 m) water samples collected over a 2-day diel period encompassing episodic weather and bloom events. Preliminary taxonomic annotations and read mappings revealed that<italic>Microcystis</italic>spp. accounted for up to ~47% of the transcriptionally active community.</p></sec>]]></ab></abstract> </profileDesc> </teiHeader> <text><body xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink"> <div xmlns="http://www.tei-c.org/ns/1.0"><p>concatenated and assembled using MEGAHIT (v.1.2.9) <ref type="bibr">(14)</ref>. Statistical assessment of coassembly quality was performed via QUAST (v.5.2.0) (15) (Table <ref type="table">1</ref>).</p><p>Preliminary mappings of libraries to the coassembly via bbmap.sh indicated that Microcystis spp. genera comprised ≤47% of the transcriptionally active community (taxonomic annotations via Kraken2) (v.2.1.3) (16) (Table <ref type="table">1</ref>). The Aphanizomenonaceae family (containing Aphanizomenon and Dolichospermum genera, which are taxonomi cally unresolved) comprised ≤8% (APH). Blastp of APH proteins against the NCBI nr database identified 4.3% as Aphanizomenon, 21% as Dolichospermum, and 74.7% as cyanobacterial/environmental. Considering that climate change is exacerbating both cyanobacterial blooms <ref type="bibr">(17,</ref><ref type="bibr">18)</ref> and episodic events <ref type="bibr">(8,</ref><ref type="bibr">19)</ref>, this data set serves as a resource to investigate how cyanobacterial communities will respond to a climatically altered future. Announcement Microbiology Resource Announcements Month XXXX Volume 0 Issue 0 10.1128/mra.00659-24 3 Downloaded from <ref type="url">https://journals.asm.org/journal/mra</ref> on 14 October 2024 by 160.36.43.139.</p></div></body> </text> </TEI>