Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system
Title: Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system
ABSTRACT Here, we report the draft genome ofAureococcus anophagefferensstrain CCMP1851, which is susceptible to the virusKratosvirus quantuckense. CCMP1851 complements an available genome for a virus-resistant strain (CCMP1850) isolated from the same bloom. Future studies can now use this genome to examine genetic hints of virus resistance and susceptibility. more »« less
Perez, Ryan King; Chavez_Rios, Jocelyn Sofia; Grifaldo, Jessica; Regner, Kurt; Pedraza-Reyes, Mario; Robleto, Eduardo A
(, Microbiology Resource Announcements)
Roux, Simon
(Ed.)
ABSTRACT We report the genome sequence ofBacillus subtilisstrain YB955, a prophage-cured strain used as a model in DNA repair, bacterial physiology, and mutagenesis studies. The assembled and annotated draft genome contains 4,031 coding genes, 5 rRNAs, and 73 tRNAs. Compared to 168, YB955 has a 134,402 bp deletion.
Farrell, Morgan V; Airkin, Mina Y; Ali, Tatyana N; Altoblani, Zainalabdin S; Bowman, Chynna R; Diaz, Abigail_Anne B; Faurot, Paul F; Frausto, Joshua E; Haji, Sazan F; Hamad, Basma A; et al
(, Microbiology Resource Announcements)
Stewart, Frank J
(Ed.)
ABSTRACT Here, we report the draft genome sequence ofExiguobacteriumsp. strain MMG028, isolated from Rose Creek, San Diego, CA, USA, assembled and analyzed by undergraduate students participating in a marine microbial genomics course. A genomic comparison suggests that MMG028 is a novel species, providing a resource for future microbiology and biotechnology investigations.
Elkassas, Sabrina M; Serres, Margrethe H; Richardson, Derrick; Zhilina, Tatyana N; Huber, Julie A
(, Microbiology Resource Announcements)
Roux, Simon
(Ed.)
ABSTRACT A methanogenic archaeon was isolated from bottom sediments in the vicinity of Lake Tanatar II (Altai, Russia), an alkaline soda lake. Here we present the draft genome sequence ofMethanocalculus natronophilussp. strain Z-7105T.
Abstract TaxonomyPotato virus Xis the type‐member of the plant‐infectingPotexvirusgenus in the familyAlphaflexiviridae. Physical propertiesPotato virus X (PVX) virions are flexuous filaments 460–480 nm in length. Virions are 13 nm in diameter and have a helical pitch of 3.4 nm. The genome is approximately 6.4 kb with a 5′ cap and 3′ poly(A) terminus. PVX contains five open reading frames, four of which are essential for cell‐to‐cell and systemic movement. One protein encodes the viral replicase. Cellular inclusions, known as X‐bodies, occur near the nucleus of virus‐infected cells. HostsThe primary host is potato, but it infects a wide range of dicots. Diagnostic hosts includeDatura stramoniumandNicotiana tabacum. PVX is transmitted in nature by mechanical contact. Useful websitehttps://talk.ictvonline.org/ictv‐reports/ictv_online_report/positive‐sense‐rna‐viruses/w/alphaflexiviridae/1330/genus‐potexvirus
Garcia, Anthony_G K; Bùi, Jo Trang; Michael, Todd P; Ickert‐Bond, Stefanie M; Di_Stilio, Verónica S
(, Applications in Plant Sciences)
Abstract PremiseAs the sister clade to angiosperms, extant gymnosperms are crucial for reconstructing ancestral gene regulatory networks in seed plants. This highlights the need for model systems representing each of their distinct lineages. However, tools to quickly and effectively investigate gene function in gymnosperms are still limited due to the challenges of long life cycles and large genome sizes. Species within the xerophytic genusEphedra(Gnetales) have comparatively smaller genomes and shrubby growth habits with shorter life spans, making them better suited for greenhouse cultivation and laboratory experiments. MethodsWe implement virus‐induced gene silencing (VIGS) to manipulate gene expression inEphedra tweedieanaviaAgrobacterium‐mediated vacuum infiltration of tobacco rattle virus (TRV1 and TRV2) into seedlings. ResultsTreatment resulted in highly efficient gene silencing of theE. tweedieana PHYTOENE DESATURASE(PDS) orthologEtwPDS. The expected photobleaching phenotype was observed as early as two weeks, and lasted at least five months in stems, shoot tips, leaves, axillary meristems, and lateral branches of treated plants. DiscussionWe report on virus‐induced targeted gene silencing ofPDSin a Gnetales representative to further enable functional studies of the genetic mechanisms underpinning adaptations in gymnosperms, an important and underrepresented lineage of seed plants.
Chase, Emily E, Truchon, Alexander R, Schepens, William W, and Wilhelm, Steven W. Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system. Retrieved from https://par.nsf.gov/biblio/10556266. Microbiology Resource Announcements 13.6 Web. doi:10.1128/mra.00292-24.
Chase, Emily E, Truchon, Alexander R, Schepens, William W, & Wilhelm, Steven W. Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system. Microbiology Resource Announcements, 13 (6). Retrieved from https://par.nsf.gov/biblio/10556266. https://doi.org/10.1128/mra.00292-24
Chase, Emily E, Truchon, Alexander R, Schepens, William W, and Wilhelm, Steven W.
"Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system". Microbiology Resource Announcements 13 (6). Country unknown/Code not available: American Society for Microbiology. https://doi.org/10.1128/mra.00292-24.https://par.nsf.gov/biblio/10556266.
@article{osti_10556266,
place = {Country unknown/Code not available},
title = {Aureococcus anophagefferens strain CCMP1851: draft genome of a second Kratosvirus quantuckense- susceptible host strain for an emerging host–giant virus model system},
url = {https://par.nsf.gov/biblio/10556266},
DOI = {10.1128/mra.00292-24},
abstractNote = {ABSTRACT Here, we report the draft genome ofAureococcus anophagefferensstrain CCMP1851, which is susceptible to the virusKratosvirus quantuckense. CCMP1851 complements an available genome for a virus-resistant strain (CCMP1850) isolated from the same bloom. Future studies can now use this genome to examine genetic hints of virus resistance and susceptibility.},
journal = {Microbiology Resource Announcements},
volume = {13},
number = {6},
publisher = {American Society for Microbiology},
author = {Chase, Emily E and Truchon, Alexander R and Schepens, William W and Wilhelm, Steven W},
editor = {Hudson, André O}
}
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