An official website of the United States government
Since the first phylogenetic study of the order Batrachospermales,Batrachospermumwas shown to be paraphyletic. Subsequently, sections of the genus have been methodically investigated usingDNAsequences and morphology in order to propose new genera and delineate species.BatrachospermumsectionTurfosais the last section with multiple species yet to be examined. New sequence data of specimens from Europe and the United States were combined with the sparse sequence data already available. Phylogenetic analyses usingrbcL andCOI‐5P sequences showed this section to be a well‐supported clade, distinct fromBatrachospermumsectionBatrachospermumand its segregate genera. Section Turfosais raised to the generic rank asPaludicolagen. nov. Substantial genetic variation within the genus was discovered and 12 species are recognized based onDNAsequence data as well as morphological characters and geographic distribution. The following morphological characters were applied to distinguish species: branching pattern (pseudodichotomous or irregular), whorl size (reduced or well developed), primary fascicles (curved or straight), spermatangia origin (primary or secondary fascicles), and carposporophyte arrangement (loose or dense). Previously published species were transferred to the new genus:P. turfosa,P. keratophyta,P. orthosticha,P. phangiae,andP. periploca. Seven new species are proposed as follows:P. groenbladiifrom Europe;P. communis,P. johnhallii, andP. leafensisfrom North America; andP. aquanigra,P. diamantinensis, andP. turfosiformisfrom Brazil. In addition, three unsequenced species in the section,P. bakarensis,P. gombakensis, andP. tapirensis, were transferred to the new genus.
more »
« less
This content will become publicly available on November 21, 2025
C3-Chlorination of C2-substituted benzo[ b ]thiophene derivatives in the presence of sodium hypochlorite
Benzo[b]thiophene was efficiently chlorinated at the C3-position using sodium hypochlorite pentahydrate. A mechanism was proposed and probed using a combination of DFT calculations and experiments.
more »
« less
- Award ID(s):
- 1954639
- PAR ID:
- 10558695
- Publisher / Repository:
- The Royal Society of Chemistry
- Date Published:
- Journal Name:
- Organic & Biomolecular Chemistry
- Volume:
- 22
- Issue:
- 45
- ISSN:
- 1477-0520
- Page Range / eLocation ID:
- 8958 to 8966
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
Context.Dark gamma-ray bursts (GRBs) constitute a significant fraction of the GRB population. In this paper, we present a multi-wavelength analysis (both prompt emission and afterglow) of an intense (3.98 × 10−5erg cm−2usingFermi-Gamma-Ray Burst Monitor) two-episodic GRB 150309A observed early on until ∼114 days post burst. Despite the strong gamma-ray emission, no optical afterglow was detected for this burst. However, we discovered near-infrared (NIR) afterglow (KS-band), ∼5.2 h post burst, with the CIRCE instrument mounted at the 10.4 m Gran Telescopio Canarias (hereafter, GTC). Aims.We aim to examine the characteristics of GRB 150309A as a dark burst and to constrain other properties using multi-wavelength observations. Methods.We usedFermiobservations of GRB 150309A to understand the prompt emission mechanisms and jet composition. We performed early optical observations using the BOOTES robotic telescope and late-time afterglow observations using the GTC. A potential faint host galaxy was also detected in the optical wavelength using the GTC. We modelled the potential host galaxy of GRB 150309A in order to explore the environment of the burst. Results.The time-resolved spectral analysis ofFermidata indicates a hybrid jet composition consisting of a matter-dominated fireball and magnetic-dominated Poynting flux. The GTC observations of the afterglow revealed that the counterpart of GRB 150309A was very red, withH − KS > 2.1 mag (95% confidence). The red counterpart was not discovered in any bluer filters ofSwiftUVOT/BOOTES, which would be indicative of a high redshift origin. Therefore we discarded this possibility based on multiple arguments, such as spectral analysis of the X-ray afterglow constrainz < 4.15 and a moderate redshift value obtained using the spectral energy distribution (SED) modelling of the potential galaxy. The broadband (X-ray to NIR bands) afterglow SED implies a very dusty host galaxy with a deeply embedded GRB (suggestingAV ≳ 35 mag). Conclusions.The environment of GRB 150309A demands a high extinction towards the line of sight. Demanding dust obscuration is the most probable origin of optical darkness as well as the very red afterglow of GRB 150309A. This result establishes GRB 150309A as the most extinguished GRB known to date.more » « less
-
Abstract Sulfoximines are popular scaffolds in drug discovery due to their hydrogen bonding properties and chemical stability. In recent years, the role of reactive intermediates such as nitrenes has been studied in the synthesis and degradation of sulfoximines. In this work, the photochemistry ofN‐phenyl dibenzothiophene sulfoximine [5‐(phenylimino)‐5H‐5λ4‐dibenzo[b,d]thiopheneS‐oxide] was analyzed. The structure resembles a combination ofN‐phenyl iminodibenzothiophene and dibenzothiopheneS‐oxide, which generate nitrene and O(3P) upon UV‐A irradiation, respectively. The photochemistry ofN‐phenyl dibenzothiophene sulfoximine was explored by monitoring the formation of azobenzene, a photoproduct of triplet nitrene, using direct irradiation and sensitized experiments. The reactivity profile was further studied through direct irradiation experiments in the presence of diethylamine (DEA) as a nucleophile. The studies demonstrated thatN‐phenyl dibenzothiophene sulfoximine underwent S–N photocleavage to release singlet phenyl nitrene which formed a mixture of azepines in the presence of DEA and generated moderate amounts of azobenzene in the absence of DEA to indicate formation of triplet phenyl nitrene.more » « less
-
Summary Despite their key phylogenetic position and their unique biology, hornworts have been widely overlooked. Until recently there was no hornwort model species amenable to systematic experimental investigation.Anthoceros agrestishas been proposed as the model species to study hornwort biology.We have developed anAgrobacterium‐mediated method for the stable transformation ofA. agrestis, a hornwort model species for which a genetic manipulation technique was not yet available.High transformation efficiency was achieved by using thallus tissue grown under low light conditions. We generated a total of 274 transgenicA. agrestislines expressing the β‐glucuronidase (GUS), cyan, green, and yellow fluorescent proteins under control of the CaMV 35S promoter and several endogenous promoters. Nuclear and plasma membrane localization with multiple color fluorescent proteins was also confirmed.The transformation technique described here should pave the way for detailed molecular and genetic studies of hornwort biology, providing much needed insight into the molecular mechanisms underlying symbiosis, carbon‐concentrating mechanism, RNA editing and land plant evolution in general.more » « less
-
Henkin, Tina M (Ed.)ABSTRACT Whole genome sequencing has revealed that the genome ofStaphylococcus aureuspossesses an uncharacterized 5-gene operon (SAOUHSC_00088–00092 in strain 8325 genome) that encodes factors with functions related to polysaccharide biosynthesis and export, indicating the existence of a new extracellular polysaccharide species. We designate this locus assscfor staphylococcal surface carbohydrate. We found that thesscgenes were weakly expressed and highly repressed by the global regulator MgrA. To characterize Ssc, Ssc was heterologously expressed inEscherichia coliand extracted by heat treatment. Ssc was also conjugated to AcrA fromCampylobacter jejuniinE. coliusing protein glycan coupling technology (PGCT). Analysis of the heat-extracted Ssc and the purified Ssc-AcrA glycoconjugate by tandem mass spectrometry revealed that Ssc is likely a polymer consisting ofN-acetylgalactosamine. We further demonstrated that the expression of thesscgenes inS. aureusaffected phage adsorption and susceptibility, suggesting that Ssc is surface-exposed. IMPORTANCESurface polysaccharides play crucial roles in the biology and virulence of bacterial pathogens.Staphylococcus aureusproduces four major types of polysaccharides that have been well-characterized. In this study, we identified a new surface polysaccharide containing N-acetylgalactosamine (GalNAc). This marks the first report of GalNAc-containing polysaccharide inS. aureus. Our discovery lays the groundwork for further investigations into the chemical structure, surface location, and role in pathogenesis of this new polysaccharide.more » « less
