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Summary In maize, 24‐nt phased, secondary small interfering RNAs (phasiRNAs) are abundant in meiotic stage anthers, but their distribution and functions are not precisely known.Using laser capture microdissection, we analyzed tapetal cells, meiocytes and other somatic cells at several stages of anther development to establish the timing of 24‐PHASprecursor transcripts and the 24‐nt phasiRNA products.By integrating RNA and small RNA profiling plus single‐molecule and small RNA FISH (smFISH or sRNA‐FISH) spatial detection, we demonstrate that the tapetum is the primary site of 24‐PHASprecursor andDcl5transcripts and the resulting 24‐nt phasiRNAs. Interestingly, 24‐nt phasiRNAs accumulate in all cell types, with the highest levels in meiocytes, followed by tapetum.Our data support the conclusion that 24‐nt phasiRNAs are mobile from tapetum to meiocytes and to other somatic cells. We discuss possible roles for 24‐nt phasiRNAs in anther cell types.
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Circular RNA oligonucleotides: enzymatic synthesis and scaffolding for nanoconstruction
Circular RNAs (∼16−44 nt) were enzymatically synthesized efficientlyviaa novel DNA dumbbell splinting strategy, further, the circular 44 nt RNA was used as scaffold strands to construct hybrid and pure RNA double crossover tiles and nanostructures.
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- Award ID(s):
- 2025187
- PAR ID:
- 10560159
- Publisher / Repository:
- The Royal Society of Chemistry
- Date Published:
- Journal Name:
- Nanoscale Horizons
- Volume:
- 9
- Issue:
- 10
- ISSN:
- 2055-6756
- Page Range / eLocation ID:
- 1749 to 1755
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/d4nh00236a
