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Imaging depth of field is shallow in applications with high magnification and high numerical aperture, such as microscopy, resulting in images with in- and out-of-focus regions. Therefore, methods to extend depth of field are of particular interest. Researchers have previously shown the advantages of using freeform components to extend depth of field, with each optical system requiring a specially designed phase plate. In this paper we present a method to enable extended depth-of-field imaging for a range of numerical apertures using freeform phase plates to create variable cubic wavefronts. The concept is similar to an Alvarez lens which creates variable spherical wavefronts through the relative translation of two transmissive elements with XY polynomial surfaces. We discuss design and optimization methods to enable extended depth of field for lenses with different numerical aperture values by considering through-focus variation of the point spread function and compare on- and off-axis performance through multiple metrics.
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All-reflective freeform microscope objective for ultra-broadband microscopy
Conventional refractive microscope objective lenses have limited applicability to a range of imaging modalities due to the dispersive nature of their optical elements. Designing a conventional refractive microscope objective that provides well-corrected imaging over a broad spectral range can be challenging, if not impossible. In contrast, reflective optics are inherently achromatic, so a system composed entirely of reflective elements is free from chromatic aberrations and, as a result, can image over an ultra-wide spectral range with perfect color correction. This study explores the design space of unobscured high numerical aperture, all-reflective microscope objectives. In particular, using freeform optical elements we obviate the need for a center obscuration, rendering the objective’s modulation transfer function comparable to that of refractive lens systems of similar numerical aperture. We detail the design process of the reflective objective, from determining the design specifications to the system optimization and sensitivity analysis. The outcome is an all-reflective freeform microscope objective lens with a 0.65 numerical aperture that provides diffraction-limited imaging and is compatible with the geometric constraints of commercial microscope systems.
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- PAR ID:
- 10560676
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Express
- Volume:
- 32
- Issue:
- 27
- ISSN:
- 1094-4087; OPEXFF
- Format(s):
- Medium: X Size: Article No. 47893
- Size(s):
- Article No. 47893
- Sponsoring Org:
- National Science Foundation
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