A MEMS‐based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including
A new high-sensitivity, low-cost, Surface Enhanced Raman Spectroscopy (SERS) sensor allows for the rapid multiplex detection of foodborne pathogens in raw poultry. Self-assembled microspheres are used to pattern a hexagonal close-packed array of nanoantennas onto a side-polished multimode fiber core. Each microsphere focuses UV radiation to a photonic nanojet within a layer of photoresist on the fiber which allows the nanoantenna geometry to be controlled. Optimizing the geometry for the excitation layer generates electric field concentrations− referred to as a hotspot− within the analyte, thereby maximizing the Raman signal and improving the signal-to-noise ratio. The side polished configuration with a larger surface area has significantly better performance than the SERS sensor on the fiber tip. The use of additive manufacturing for the fiber polishing jigs as well as the sample testing compartment simplifies the sensor development and testing. Experimental results demonstrate a sensitivity range of 0.4–0.5 cells/ml achieved using raw chicken rinsates spiked with
- PAR ID:
- 10561712
- Publisher / Repository:
- Nature Publishing Group
- Date Published:
- Journal Name:
- Microsystems & Nanoengineering
- Volume:
- 10
- Issue:
- 1
- ISSN:
- 2055-7434
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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