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5-ethynyl uridine labeling of nascent mitochondrial genome transcription v1
This protocol details a method for 5-ethynyl uridine labeling of nascent mitochondrial genome transcription coupled with immunofluorescence detection of mtDNA-binding proteins in cultured cells. The result is a fluorescent readout of localized mitochondrial gene transcription compatible with high-resolution microscopy.
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- Award ID(s):
- 2339182
- PAR ID:
- 10569735
- Publisher / Repository:
- Springer Nature
- Date Published:
- Edition / Version:
- 1
- Subject(s) / Keyword(s):
- Fluorescence microscopy, Transcription, mitochondria, mtDNA, click chemistry
- Format(s):
- Medium: X
- Institution:
- University of California, Berkeley
- Sponsoring Org:
- National Science Foundation
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