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This publication documents 3D image stacks from HR-pQCT imaging of a femur diaphysis, as well as image stacks for two in-situ loaded fracture mechanics specimens observed with 3D X-ray microscopy. Imaging For HR-qQCT: HR-pQCT scans were acquired by Rachel Surowiec using an XtremeCT II scanner (SCANCO Medical AG, Bruttisellen, Switzerland) within the Musculoskeletal Function, Imaging and Tissue (MSK-FIT) Resource Core of the Indiana Center for Musculoskeletal Health’s Clinical Research Center (Indiana University, Indianapolis, IN). Scans are performed at 60.7 um resolution, a 68 kV, 1467 uA, 43 ms integration time, 1 frame averaging. Raw scans are ‘.RSQ’ file types. The ISQ file type were read into ImageJ using the Import-KHKs Scanco uCT ISQ file reader plug-in, and exported as bmp image stacks, image stacks are provided in two parts. Reconstructed images are rotated in dataviewer so that all bones are in the same orientation (prox/distal/anterior/posterior for the Femur). For in-situ fracture mechanics experiments: 3D scans were acquired by Glynn Gallaway using a 3-point bending rig for single edged notched bend specimens with a Deben CT5000N load cell (Deben, Bury St. Edmunds, UK) in a Zeiss XRADIA 510 Versa 3D X-Ray microscope (Carl Zeiss AG, Baden-Württemberg, Germany) at Purdue University. The 3-point bending frame had a span 20 mm with X-ray transparent, glassy carbon supports. To maintain hydration, the beam was wrapped in a plastic film slit at the notch. Displacements were applied at 0.1 mm/min. Load cell outputs were monitored and recorded. Displacements are held constant during image acquisitions. The first 3D image was obtained at the onset of non-linearity. Subsequently, the displacement was increased until a load increase of 10 N was observed, and another image was obtained. This sequence was repeated 6-times until peak load. 3D X-ray images were acquired with a resolution of 4.5 um, exposure time 5 sec., 801 projections, 120 kV, 10 W, 4 x objective, and a LE2 filter. X-ray projections were processed through XRADIA Scout-and-Scan Reconstructor. A recursive Gaussian smoothing filter (s=1 pixel) was applied to reduce image artifacts. Image stacks are exported as tiff files and provided individually for each load step and specimen. Two experiments are documented (beam 1 and beam 2). MaterialstThe diaphysis of a human (92-year-old, male) cadaveric femur was obtained through the Indiana University School of Medicine Anatomical Donation Program.
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Image Data and Analysis Materials for In-situ Fracture Mechanics Experiments on Human Cortical Bone Treated Ex-vivo with Raloxifene
This publication documents 3D image stacks for two in-situ loaded fracture mechanics specimens observed with 3D X-ray microscopy, 2D image stacks of the crack mouth opening displacement during said loading, and analysis codes which supported the publication. Materials: The diaphysis of a human (75-year-old, male) cadaveric femur was obtained through the Indiana University School of Medicine Anatomical Donation Program. Nominal 4 mm x 4 mm x 24 mm beams were sectioned from the femur diaphysis. Experiments were conducted on 12 beams. Beams were assigned to 2 groups: treated with Raloxifene (RAL), treated with a vehicle (VEH) control. Image data are provided for one beam from the RAL group and one beam from the VEH group. Additional images can be made available upon request to the corresponding author. Imaging: ;For in-situ fracture mechanics experiments: 2D and 3D scans were acquired by Glynn Gallaway using a 4-point bending rig for single edged notched bend specimens in a water bath with a Deben CT5000N load cell (Deben, Bury St. Edmunds, UK) in a Zeiss XRADIA 510 Versa 3D X-Ray microscope (Carl Zeiss AG, Baden-Wuerttemberg, Germany) at Purdue University. The 4-point bending frame had a span 16 mm with X-ray transparent, glassy carbon supports. To maintain hydration, bending frame was situated in a waterbath filled with DI water. Displacements were applied at 0.1 mm/min. Load cell outputs were monitored and recorded. During loading 2D images were obtained every 1 second. 3D X-ray images were acquired with a resolution of 4.5 um, exposure time 6 sec., 2401 projections, 120 kV, 10 W, 4 x objective, and a LE2 filter. X-ray projections were processed through XRADIA Scout-and-Scan Reconstructor. A recursive Gaussian smoothing filter (1 pixel) was applied to reduce image artifacts. Image stacks are exported as tiff files and provided for each specimen. Images are provided for one RAL treated sample and one VEH treated sample.
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- Award ID(s):
- 1952993
- PAR ID:
- 10583055
- Publisher / Repository:
- Purdue University Research Repository
- Date Published:
- Subject(s) / Keyword(s):
- Bone Mechanical Engineering Mechanical engineering Imaging Fracture Cracks Raloxifene Mechanical engineering
- Format(s):
- Medium: X Size: 8 files; 2 GB Other: application/zip; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet; text/plain; image/jpeg
- Size(s):
- 8 files 2 GB
- Location:
- Purdue University Research Repository (PURR)
- Right(s):
- Creative Commons Zero v1.0 Universal
- Institution:
- Purdue University
- Sponsoring Org:
- National Science Foundation
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