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Title: Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography
Candida boidiniiNAD+-dependent formate dehydrogenase (CbFDH) has gained significant attention for its potential application in the production of biofuels and various industrial chemicals from inorganic carbon dioxide. The present study reports the atomic X-ray crystal structures of wild-typeCbFDH at cryogenic and ambient temperatures, as well as that of the Val120Thr mutant at cryogenic temperature, determined at the Turkish Light Source `Turkish DeLight'. The structures reveal new hydrogen bonds between Thr120 and water molecules in the active site of the mutantCbFDH, suggesting increased stability of the active site and more efficient electron transfer during the reaction. Further experimental data is needed to test these hypotheses. Collectively, these findings provide invaluable insights into future protein-engineering efforts that could potentially enhance the efficiency and effectiveness ofCbFDH.  more » « less
Award ID(s):
1231306
PAR ID:
10587057
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Acta Crystallographica Section D Structural Biology
Date Published:
Journal Name:
Acta Crystallographica Section D Structural Biology
Volume:
79
Issue:
11
ISSN:
2059-7983
Page Range / eLocation ID:
1010 to 1017
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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