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Title: High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source
Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A2Aadenosine receptor (A2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.  more » « less
Award ID(s):
1231306
PAR ID:
10588795
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; more » ; « less
Publisher / Repository:
IUCrJ
Date Published:
Journal Name:
IUCrJ
Volume:
6
Issue:
3
ISSN:
2052-2525
Page Range / eLocation ID:
412 to 425
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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