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We report the design conception, chemical synthesis, and microbiological evaluation of the bridged macrobicyclic antibiotic cresomycin (CRM), which overcomes evolutionarily diverse forms of antimicrobial resistance that render modern antibiotics ineffective. CRM exhibits in vitro and in vivo efficacy against both Gram-positive and Gram-negative bacteria, including multidrug-resistant strains ofStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosa. We show that CRM is highly preorganized for ribosomal binding by determining its density functional theory–calculated, solution-state, solid-state, and (wild-type) ribosome-bound structures, which all align identically within the macrobicyclic subunits. Lastly, we report two additional x-ray crystal structures of CRM in complex with bacterial ribosomes separately modified by the ribosomal RNA methylases, chloramphenicol-florfenicol resistance (Cfr) and erythromycin-resistance ribosomal RNA methylase (Erm), revealing concessive adjustments by the target and antibiotic that permit CRM to maintain binding where other antibiotics fail.
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This content will become publicly available on May 15, 2026
Non-linear, Team-based VR Training for Cardiac Arrest Care with enhanced CRM Toolkit
This paper introduces iREACT, a novel VR simulation addressing key limitations in traditional cardiac arrest (CA) training. Conventional methods struggle to replicate the dynamic nature of real CA events, hindering Crew Resource Management (CRM) skill development. iREACT provides a non-linear, collaborative environment where teams respond to changing patient states, mirroring real CA complexities. By capturing multi-modal data (user actions, cognitive load, visual gaze) and offering real-time and post-session feedback, iREACT enhances CRM assessment beyond traditional methods. A formative evaluation with medical experts underscores its usability and educational value, with potential applications in other high-stakes training scenarios to improve teamwork, communication, and decision-making.
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- Award ID(s):
- 2202451
- PAR ID:
- 10590942
- Editor(s):
- Ceylan, Duygu; Li, Tzu-Mao
- Publisher / Repository:
- The Eurographics Association
- Date Published:
- Journal Name:
- Eurographics technical report series
- ISSN:
- 1017-4656
- ISBN:
- 978-3-03868-268-4
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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