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Title: miR-31-mediated local translation at the mitotic spindle is important for early development
ABSTRACT miR-31 is a highly conserved microRNA that plays crucial roles in cell proliferation, migration and differentiation. We discovered that miR-31 and some of its validated targets are enriched on the mitotic spindle of the dividing sea urchin embryo and mammalian cells. Using the sea urchin embryo, we found that miR-31 inhibition led to developmental delay correlated with increased cytoskeletal and chromosomal defects. We identified miR-31 to directly suppress several actin remodeling transcripts, including β-actin, Gelsolin, Rab35 and Fascin. De novo translation of Fascin occurs at the mitotic spindle of sea urchin embryos and mammalian cells. Importantly, miR-31 inhibition leads to a significant a increase of newly translated Fascin at the spindle of dividing sea urchin embryos. Forced ectopic localization of Fascin transcripts to the cell membrane and translation led to significant developmental and chromosomal segregation defects, highlighting the importance of the regulation of local translation by miR-31 at the mitotic spindle to ensure proper cell division. Furthermore, miR-31-mediated post-transcriptional regulation at the mitotic spindle may be an evolutionarily conserved regulatory paradigm of mitosis.  more » « less
Award ID(s):
2103453
PAR ID:
10594322
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ;
Publisher / Repository:
The Company of Biologists Ltd |
Date Published:
Journal Name:
Development
Volume:
151
Issue:
17
ISSN:
0950-1991
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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