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The Drosophila kikkawai feature with NCBI Gene ID 108084518 was determined to be an ortholog of Drosophila melanogaster Sox102F, a member of the FlyBase High Mobility Group Box Transcription Factors gene group (FBgg0000748). Five isoforms were constructed using the GEP F element annotation protocol, the longest being novel isoform Sox102F-PNE (identified using the XM_017180752 RefSeq prediction and RNA-seq data). Among the isoforms found in both D. melanogaster and D. kikkawai, Sox102F-PB is the longest and exhibits a 1.18x coding span expansion due to transposable element insertion into an intron. All D. kikkawai protein isoforms contain the conserved domain HMG_box_dom (IPR009071).
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Drosophila kikkawai ortholog of the D. melanogaster Muller D element ash1
The Drosophila kikkawai feature with Gene ID 108083276 was determined to be an ortholog of Drosophila melanogaster absent, small, or homeotic discs 1 (ash1). Two isoforms, ash1-PB and ash1-PC, were constructed on the D. kikkawai Muller D element using the GEP annotation protocol. The second coding exon of D. kikkawai ash1 includes an insertion translated into 18 additional amino acids compared to the D. melanogaster protein and is supported by RNA-Seq coverage, the lack of splice junction predictions, and multiple gene predictors. The first intron in both isoforms of D. kikkawai ash1 contains a well conserved non-canonical GC splice site.
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- Award ID(s):
- 2114661
- PAR ID:
- 10598073
- Publisher / Repository:
- microPublication Biology
- Date Published:
- Journal Name:
- microPublication biology
- Volume:
- 2024
- ISSN:
- 2578-9430
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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