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This content will become publicly available on January 1, 2026

Title: Epitope tags are not created equal: Disruption of cellular function of a translation factor by a short viral tag
Cellular identity and fate are determined by the proteins synthesized. Initiation of mRNA translation requires an important translation factor, eIF4G (ifg-1 in C. elegans). Embryos use mRNA translational control for spatial and temporal regulation of protein synthesis. Using CRISPR engineering, we added in-frame epitope and fluorescent tags (V5, Myc, Flag, GFP, and mCherry) to IFG-1. Tagged forms containing the V5 epitope caused embryonic arrest. Internal disruption of the V5 tag restored viability at 25°C. This study demonstrates that the molecular nature of a small epitope tag is sufficient to disrupt C. elegans embryogenesis.  more » « less
Award ID(s):
2119959
PAR ID:
10599134
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
microPublication Biology
Date Published:
Journal Name:
microPublication biology
Volume:
2025
ISSN:
2578-9430
Subject(s) / Keyword(s):
translation initiation factor embryogenesis Crispr engineering tagged proteins
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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