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Title: High‐Efficiency PET Degradation With a Duo‐Enzyme System Immobilized on Magnetic Nanoparticles
The widespread consumption of PET worldwide has necessitated the search for environment‐friendly methods for PET degradation and recycling. Among these methods, biodegradation stands out as a promising approach for recycling PET. The discovery of duo enzyme system PETase and MHETase in 2016, along with their engineered variants, has demonstrated significant potential in breaking down PET. Previous studies have also demonstrated that the activity of the enzyme PETase increases when it is immobilized on nanoparticles. To achieve highly efficient and complete PET depolymerization, we immobilized both FAST‐PETase and MHETase at a specific ratio on magnetic nanoparticles. This immobilization resulted in a 2.5‐fold increase in product release compared with free enzymes. Additionally, we achieved reusability and enhanced stability of the enzyme bioconjugates.  more » « less
Award ID(s):
2132156
PAR ID:
10635887
Author(s) / Creator(s):
; ;
Publisher / Repository:
Wiley
Date Published:
Journal Name:
Biotechnology and Bioengineering
Volume:
122
Issue:
6
ISSN:
0006-3592
Page Range / eLocation ID:
1397 to 1401
Subject(s) / Keyword(s):
Co-immobilizationl bioconjugate formation PET degradation nanoparticle enzyme assembly
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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