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SUMMARY Extracellular ATP (eATP) signaling inArabidopsis thalianais mediated by the purinoceptor P2K1. Previous studies have clarified that the downstream transcriptional responses to eATP involve jasmonate (JA)‐based signaling components such as the JA receptor (COI1) and JA‐responsive bHLH transcription factors (MYCs). However, the specific contributions of JA itself on eATP signaling are unexplored. Here, we report that JA primes plant responses to eATP through P2K1. Our findings show that JA treatment significantly upregulatesP2K1transcription, corroborating our observation that JA facilitates eATP‐induced cytosolic calcium elevation and transcriptional reprogramming in a JA signaling‐dependent manner. Additionally, we find that salicylic acid pre‐treatment represses eATP‐induced plant response. These results suggest that JA accumulation during biotic or abiotic stresses potentiates eATP signaling, enabling plants to better cope with subsequent stress events.
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This content will become publicly available on September 24, 2026
Jasmonate Primes Plant Responses to Extracellular ATP
Extracellular ATP (eATP) signaling inArabidopsis thalianais mediated by the purinoceptor P2K1. Previous studies have clarified that the downstream transcriptional responses to eATP involve jasmonate (JA)-based signaling components such as the JA receptor (COI1) and JA-responsive bHLH transcription factors (MYCs). However, the specific contributions of JA signaling itself on eATP signaling are unexplored. Here, we report that JA primes plant responses to eATP through P2K1. Our findings show that JA treatment significantly upregulatesP2K1transcription, corroborating our observation that JA facilitates eATP-induced cytosolic calcium elevation and transcriptional reprogramming in a JA signaling-dependent manner. Additionally, we find that salicylic acid pretreatment represses eATP-induced plant response. These results suggest that JA accumulation during biotic or abiotic stresses may potentiate eATP signaling, enabling plants to better cope with subsequent stress events. Plant hormone jasmonate (JA) enhances plant responses to extracellular ATP (eATP) inArabidopsis thalianathrough a mechanism dependent on the JA receptor COI1 and the eATP receptor P2K1. The reciprocal amplification of these signals provides a mechanistic explanation for how plants effectively respond to different stress events.
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- Award ID(s):
- 2048410
- PAR ID:
- 10637940
- Publisher / Repository:
- The Plant Journal
- Date Published:
- Subject(s) / Keyword(s):
- jasmonate, innate immunity, signaling, receptor like kinase
- Format(s):
- Medium: X
- Institution:
- bioRxiv
- Sponsoring Org:
- National Science Foundation
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