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CRISPR-Cas9 is revolutionizing how we conduct scientific research, treat disease, and develop new crops. The widespread impact of this genome-editing technology makes it critical for undergraduate students to understand and engage with this new tool. In this article, we describe a multi-week lab module that teaches undergraduates how to design CRISPR-Cas9 constructs and screen for CRISPR-modified genotypes. The module is conducted through the lens of independent research; students conduct a genotype screen for novel knockout mutations. In our module, students screen Zea mays (maize) seedlings for mutations in the MAD2 gene, which assists our ongoing investigation of meiotic chromosome segregation. This module can be adapted to knockout any gene in any organism, and thus align with an instructor’s research program. Engaging in original research helps undergraduate students develop independence and initiative in the lab as well as the molecular techniques of CRISPR-Cas9.
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This content will become publicly available on February 22, 2026
Introduction to CRISPR-Cas9 Techniques: Strategies for the Laboratory and the Classroom
This open-access textbook provides an in-depth introduction to CRISPR-Cas9 technology and explores its use across the gamut of biological model systems. This volume is intended for classroom instructors at the high school and undergraduate level. It provides insight into integration of CRISPR-Cas into undergraduate laboratory courses as well as options for dry-bench work and activities to scaffold learning.
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- Award ID(s):
- 2120417
- PAR ID:
- 10643883
- Publisher / Repository:
- Springer
- Date Published:
- Edition / Version:
- 1
- ISSN:
- 2509-6133
- ISBN:
- 978-3-031-73734-3
- Page Range / eLocation ID:
- doi.org/10.1007/978-3-031-73734-3
- Subject(s) / Keyword(s):
- open access understanding CRISPR-Cas CRISPR-Cas model systems CRSIPR-Cas education Ethics and CRISPR-Cas, CRISPR-Cas applications CRISPR-Cas in the classroom Teaching CRISPR-Cas
- Format(s):
- Medium: X Size: 14896 KB Other: PDF
- Size(s):
- 14896 KB
- Sponsoring Org:
- National Science Foundation
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