%AArbelaez, Juan [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%AArbelaez, Juan [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%AMaron, Lyza [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%AMaron, Lyza [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%AJobe, Timothy [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA, Boyce Thompson Institute Cornell University Ithaca NY USA]%AJobe, Timothy [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA; Boyce Thompson Institute; Cornell University; Ithaca NY USA]%APiñeros, Miguel [Robert W. Holley Center for Agriculture and Health USDA‐ARS Cornell University Ithaca NY USA]%APiñeros, Miguel [Robert W. Holley Center for Agriculture and Health; USDA-ARS; Cornell University; Ithaca NY USA]%AFamoso, Adam [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%AFamoso, Adam [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%ARebelo, Ana [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%ARebelo, Ana [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%ASingh, Namrata [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%ASingh, Namrata [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%AMa, Qiyue [Boyce Thompson Institute Cornell University Ithaca NY USA]%AMa, Qiyue [Boyce Thompson Institute; Cornell University; Ithaca NY USA]%AFei, Zhangjun [Boyce Thompson Institute Cornell University Ithaca NY USA]%AFei, Zhangjun [Boyce Thompson Institute; Cornell University; Ithaca NY USA]%AKochian, Leon [Robert W. Holley Center for Agriculture and Health USDA‐ARS Cornell University Ithaca NY USA]%AKochian, Leon [Robert W. Holley Center for Agriculture and Health; USDA-ARS; Cornell University; Ithaca NY USA]%AMcCouch, Susan [Plant Breeding and Genetics Section School of Integrative Plant Science Cornell University Ithaca NY USA]%AMcCouch, Susan [Plant Breeding and Genetics Section; School of Integrative Plant Science; Cornell University; Ithaca NY USA]%BJournal Name: Plant Direct; Journal Volume: 1; Journal Issue: 4; Related Information: CHORUS Timestamp: 2023-09-16 14:11:59 %D2017%IWiley Blackwell (John Wiley & Sons) %JJournal Name: Plant Direct; Journal Volume: 1; Journal Issue: 4; Related Information: CHORUS Timestamp: 2023-09-16 14:11:59 %K %MOSTI ID: 10046071 %PMedium: X %TALUMINUM RESISTANCE TRANSCRIPTION FACTOR1 ( ART1 ) contributes to natural variation in aluminum resistance in diverse genetic backgrounds of rice ( O. sativa ) %XAbstract

Transcription factors (TFs) regulate the expression of other genes to indirectly mediate stress resistance mechanisms. Therefore, when studyingTF‐mediated stress resistance, it is important to understand howTFs interact with genes in the genetic background. Here, we fine‐mapped the aluminum (Al) resistanceQTLAlt12.1to a 44‐kb region containing six genes. Among them isART1, which encodes a C2H2‐type zinc fingerTFrequired for Al resistance in rice. The mapping parents, Al‐resistant cv Azucena (tropical japonica) and Al‐sensitive cvIR64 (indica), have extensive sequence polymorphism within theART1coding region, but similarART1 expression levels. Using reciprocal near‐isogenic lines (NILs) we examined how allele‐swapping theAlt12.1locus would affect plant responses to Al. Analysis of global transcriptional responses to Al stress in roots of theNILs alongside their recurrent parents demonstrated that the presence of theAlt12.1from Al‐resistant Azucena led to greater changes in gene expression in response to Al when compared to theAlt12.1fromIR64 in both genetic backgrounds. The presence of theART1 allele from the opposite parent affected the expression of several genes not previously implicated in rice Al tolerance. We highlight examples where putatively functional variation incis‐regulatory regions ofART1‐regulated genes interacts withART1 to determine gene expression in response to Al. ThisART1–promoter interaction may be associated with transgressive variation for Al resistance in the Azucena ×IR64 population. These results illustrate howART1 interacts with the genetic background to contribute to quantitative phenotypic variation in rice Al resistance.

%0Journal Article