%ALee, Jung [Department of Mechanical Engineering Southern Methodist University Dallas TX USA]%ALee, Jung [Department of Mechanical Engineering; Southern Methodist University; Dallas TX USA]%APeng, Bin [Department of Mechanical Engineering Southern Methodist University Dallas TX USA]%APeng, Bin [Department of Mechanical Engineering; Southern Methodist University; Dallas TX USA]%ASabuncu, Ahmet [Department of Mechanical Engineering Southern Methodist University Dallas TX USA]%ASabuncu, Ahmet [Department of Mechanical Engineering; Southern Methodist University; Dallas TX USA]%ANam, Seungjin [Department of Materials Science and Engineering The University of Texas at Dallas Richardson TX USA, School of Advanced Materials Engineering Kookmin University Seoul Republic of Korea]%ANam, Seungjin [Department of Materials Science and Engineering; The University of Texas at Dallas; Richardson TX USA; School of Advanced Materials Engineering Kookmin University; Seoul Republic of Korea]%AAhn, ChiWon [Nano‐Materials Laboratory National NanoFab Center Daejeon Republic of Korea]%AAhn, ChiWon [Nano-Materials Laboratory; National NanoFab Center; Daejeon Republic of Korea]%AKim, Moon [Department of Materials Science and Engineering The University of Texas at Dallas Richardson TX USA]%AKim, Moon [Department of Materials Science and Engineering; The University of Texas at Dallas; Richardson TX USA]%AKim, MinJun [Department of Mechanical Engineering Southern Methodist University Dallas TX USA]%AKim, MinJun [Department of Mechanical Engineering; Southern Methodist University; Dallas TX USA]%BJournal Name: ELECTROPHORESIS; Journal Volume: 39; Journal Issue: 5-6; Related Information: CHORUS Timestamp: 2023-09-10 23:34:50 %D2017%IWiley Blackwell (John Wiley & Sons) %JJournal Name: ELECTROPHORESIS; Journal Volume: 39; Journal Issue: 5-6; Related Information: CHORUS Timestamp: 2023-09-10 23:34:50 %K %MOSTI ID: 10048172 %PMedium: X %TMultiple consecutive recapture of rigid nanoparticles using a solid‐state nanopore sensor %XAbstract

Solid‐state nanopore sensors have been used to measure the size of a nanoparticle by applying a resistive pulse sensing technique. Previously, the size distribution of the population pool could be investigated utilizing data from a single translocation, however, the accuracy of the distribution is limited due to the lack of repeated data. In this study, we characterized polystyrene nanobeads utilizing single particle recapture techniques, which provide a better statistical estimate of the size distribution than that of single sampling techniques. The pulses and translocation times of two different sized nanobeads (80 nm and 125 nm in diameter) were acquired repeatedly as nanobeads were recaptured multiple times using an automated system controlled by custom‐built scripts. The drift‐diffusion equation was solved to find good estimates for the configuration parameters of the recapture system. The results of the experiment indicated enhancement of measurement precision and accuracy as nanobeads were recaptured multiple times. Reciprocity of the recapture and capacitive effects in solid state nanopores are discussed. Our findings suggest that solid‐state nanopores and an automated recapture system can also be applied to soft nanoparticles, such as liposomes, exosomes, or viruses, to analyze their mechanical properties in single‐particle resolution.

%0Journal Article