%ACastillo-Torres, Keisha%AMcLamore, Eric%AArnold, David%BJournal Name: Micromachines; Journal Volume: 11; Journal Issue: 1 %D2020%I %JJournal Name: Micromachines; Journal Volume: 11; Journal Issue: 1 %K %MOSTI ID: 10177452 %PMedium: X %TA High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring %XThe long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as Escherichia coli, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for magnetic separation microfluidic analysis platforms that typically function with <20 µL/s throughput. Here, we report the use of 1.5-µm-diameter magnetic microdisc to selectively tag target bacteria, and a high-throughput microfluidic device that can potentially isolate the magnetically tagged bacteria from 100 mL water samples in less than 15 min. Simulations and experiments show ~90% capture efficiencies of magnetic particles at flow rates up to 120 µL/s. Also, the platform enables the magnetic microdiscs/bacteria conjugates to be directly imaged, providing a path for quantitative assay. %0Journal Article