%APausch, Patrick [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.]%AAl-Shayeb, Basem [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA.]%ABisom-Rapp, Ezra [College of Natural Resources, University of California, Berkeley, Berkeley, CA, USA.]%ATsuchida, Connor [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., University of California, Berkeley—University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA.]%ALi, Zheng [Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles, CA, USA.]%ACress, Brady [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.]%AKnott, Gavin [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia.]%AJacobsen, Steven [Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles, CA, USA., Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA.]%ABanfield, Jillian [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, USA.]%ADoudna, Jennifer [Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA., Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA., Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.]%BJournal Name: Science; Journal Volume: 369; Journal Issue: 6501; Related Information: CHORUS Timestamp: 2021-04-01 13:12:57 %D2020%IAmerican Association for the Advancement of Science (AAAS); None %JJournal Name: Science; Journal Volume: 369; Journal Issue: 6501; Related Information: CHORUS Timestamp: 2021-04-01 13:12:57 %K %MOSTI ID: 10219901 %PMedium: X %TCRISPR-CasΦ from huge phages is a hypercompact genome editor %X

CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox.

%0Journal Article