%ABrückner, David%AArlt, Nicolas%AFink, Alexandra%ARonceray, Pierre%ARädler, Joachim%ABroedersz, Chase%Anull Ed.%BJournal Name: Proceedings of the National Academy of Sciences; Journal Volume: 118; Journal Issue: 7 %D2021%I %JJournal Name: Proceedings of the National Academy of Sciences; Journal Volume: 118; Journal Issue: 7 %K %MOSTI ID: 10230576 %PMedium: X %TLearning the dynamics of cell–cell interactions in confined cell migration %XThe migratory dynamics of cells in physiological processes, ranging from wound healing to cancer metastasis, rely on contact-mediated cell–cell interactions. These interactions play a key role in shaping the stochastic trajectories of migrating cells. While data-driven physical formalisms for the stochastic migration dynamics of single cells have been developed, such a framework for the behavioral dynamics of interacting cells still remains elusive. Here, we monitor stochastic cell trajectories in a minimal experimental cell collider: a dumbbell-shaped micropattern on which pairs of cells perform repeated cellular collisions. We observe different characteristic behaviors, including cells reversing, following, and sliding past each other upon collision. Capitalizing on this large experimental dataset of coupled cell trajectories, we infer an interacting stochastic equation of motion that accurately predicts the observed interaction behaviors. Our approach reveals that interacting noncancerous MCF10A cells can be described by repulsion and friction interactions. In contrast, cancerous MDA-MB-231 cells exhibit attraction and antifriction interactions, promoting the predominant relative sliding behavior observed for these cells. Based on these experimentally inferred interactions, we show how this framework may generalize to provide a unifying theoretical description of the diverse cellular interaction behaviors of distinct cell types. %0Journal Article