%AShams, Arik%AHiggins, Sean%AFellmann, Christof%ALaughlin, Thomas%AOakes, Benjamin%ALew, Rachel%AKim, Shin%ALukarska, Maria%AArnold, Madeline%AStaahl, Brett%ADoudna, Jennifer%ASavage, David%BJournal Name: Nature Communications; Journal Volume: 12; Journal Issue: 1; Related Information: CHORUS Timestamp: 2022-12-02 10:15:59 %D2021%INature Publishing Group %JJournal Name: Nature Communications; Journal Volume: 12; Journal Issue: 1; Related Information: CHORUS Timestamp: 2022-12-02 10:15:59 %K %MOSTI ID: 10305419 %PMedium: X %TComprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules %X
Proteins evolve through the modular rearrangement of elements known as domains. Extant, multidomain proteins are hypothesized to be the result of domain accretion, but there has been limited experimental validation of this idea. Here, we introduce a technique for genetic minimization by