%AZhang, Genwei%ABrown, Joseph%AQuartararo, Anthony%ALi, Chengxi%ATan, Xuyu%AHanna, Stephanie%AAntilla, Sarah%ACowfer, Amanda%ALoas, Andrei%APentelute, Bradley%BJournal Name: Communications Chemistry; Journal Volume: 5; Journal Issue: 1; Related Information: CHORUS Timestamp: 2022-11-25 04:12:31 %D2022%INature Publishing Group %JJournal Name: Communications Chemistry; Journal Volume: 5; Journal Issue: 1; Related Information: CHORUS Timestamp: 2022-11-25 04:12:31 %K %MOSTI ID: 10361716 %PMedium: X %TRapid de novo discovery of peptidomimetic affinity reagents for human angiotensin converting enzyme 2 %X
Rapid discovery and development of serum-stable, selective, and high affinity peptide-based binders to protein targets are challenging. Angiotensin converting enzyme 2 (ACE2) has recently been identified as a cardiovascular disease biomarker and the primary receptor utilized by the severe acute respiratory syndrome coronavirus 2. In this study, we report the discovery of high affinity peptidomimetic binders to ACE2 via affinity selection-mass spectrometry (AS-MS). Multiple high affinity ACE2-binding peptides (ABP) were identified by selection from canonical and noncanonical peptidomimetic libraries containing 200 million members (dissociation constant,