Journal ArticleDetection of soil-borne wheat mosaic virus using hyperspectral imaging: from lab to field scans and from hyperspectral to multispectral dataHaagsma, Marja; Hagerty, Christina H.; Kroese, Duncan R.; Selker, John S.Abstract Hyperspectral imaging allows for rapid, non-destructive and objective assessments of crop health. Narrowband-hyperspectral data was used to select wavelength regions that can be exploited to identify wheat infected with soil-borne mosaic virus. First, leaf samples were scanned in the lab to investigate spectral differences between healthy and diseased leaves, including non-symptomatic and symptomatic areas within a diseased leaf. The potential of 84 commonly used vegetation indices to find infection was explored. A machine-learning approach was used to create a classification model to automatically separate pixels into symptomatic, non-symptomatic and healthy classes. The success rate of the model was 69.7% using the full spectrum. It was very encouraging that by using a subset of only four broad bands, sampled to simulate a data set from a much simpler and less costly multispectral camera, accuracy increased to 71.3%. Next, the classification models were validated on field data. Infection in the field was successfully identified using classifiers trained on the entire spectrum of the hyperspectral data acquired in a lab setting, with the best accuracy being 64.9%. Using a subset of wavelengths, simulating multispectral data, the accuracy dropped by only 3 percentage points to 61.9%. This research shows the potential of using lab scans to train classifiers to be successfully applied in the field, even when simultaneously reducing the hyperspectral data to multispectral data.2023-01-0110398474Precision Agriculture1385-2256https://doi.org/10.1007/s11119-022-09986-01832109; 1832170; 2243961; 2243963National Science Foundation