Journal ArticleStretchable Nanofiber-Based Felt as a String Electrode for Potential Use in Wearable Glucose BiosensorsSeufert, Bianca; Thomas, Sylvia; Takshi, Arash<p>Nanofiber technology is leading the revolution of wearable technology and provides a unique capability to fabricate smart textiles. With the novel fabrication technique of electrospinning, nanofibers can be fabricated and then manufactured into a durable conductive string for the application of smart textiles. This paper presents an electrospun nanofiber mesh-based (NF-Felt) string electrode with a conducting polymer coating for an electrochemical enzymatic glucose sensor. The surface area of a nanofiber matrix is a key physical property for enhanced glucose oxidase (GOx) enzyme binding for the development of an electrochemical biosensor. A morphological characterization of the NF-Felt string electrode was performed using scanning electron microscopy (SEM) and compared with a commercially available cotton–polyester (Cot-Pol) string coated with the same conducting polymer. The results from stress–strain testing demonstrated high stretchability of the NF-Felt string. Also, the electrochemical characterization results showed that the NF-Felt string electrode was able to detect a glucose concentration in the range between 0.0 mM and 30.0 mM with a sensitivity of 37.4 μA/mM·g and a detection limit of 3.31 mM. Overall, with better electrochemical performance and incredible flexibility, the NF-Felt-based string electrode is potentially more suitable for designing wearable biosensors for the detection of glucose in sweat.</p>Sensors2024-02-0110560142Sensors24412831424-8220https://doi.org/10.3390/s240412831952589; 1953089electrospun nanofibersflexible string electrodeswearable electronicselectrochemical glucose biosensorcyclic voltammetry (CV)electrochemical impedance spectroscopy (EIS)National Science Foundation