Journal ArticleMid-infrared hyperspectral microscopy with broadband 1-GHz dual frequency combsChang, Peter; Ishrak, Ragib; Hoghooghi, Nazanin; Egbert, Scott; Lesko, Daniel; Swartz, Stephanie; Biegert, Jens; Rieker, Gregory B; Reddy, Rohith; Diddams, Scott AMid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth, and high signal-to-noise ratio. In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers 1000 cm−1 at νc = 2941 cm−1 with 12.86 kHz spectra acquisition rate and 5 µm spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.</p>AIP Publishing2024-10-0110565682APL Photonics9102378-0967https://doi.org/10.1063/5.02256162019195; 2016244National Science Foundation