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Phage emit communication signals that inform their lytic and lysogenic life cycles. However, little is known regarding the abundance and diversity of the genes associated with phage communication systems in wastewater treatment microbial communities. This study focused on phage communities within two distinct biochemical wastewater environments, specifically aerobic membrane bioreactors (AeMBRs) and anaerobic membrane bioreactors (AnMBRs) exposed to varying antibiotic concentrations. Metagenomic data from the bench-scale systems were analyzed to explore phage phylogeny, life cycles, and genetic capacity for antimicrobial resistance and quorum sensing. Two dominant phage families, Schitoviridae and Peduoviridae, exhibited redox-dependent dynamics. Schitoviridae prevailed in anaerobic conditions, while Peduoviridae dominated in aerobic conditions. Notably, the abundance of lytic and lysogenic proteins varied across conditions, suggesting the coexistence of both life cycles. Furthermore, the presence of antibiotic resistance genes (ARGs) within viral contigs highlighted the potential for phage to transfer ARGs in AeMBRs. Finally, quorum sensing genes in the virome of AeMBRs indicated possible molecular signaling between phage and bacteria. Overall, this study provides insights into the dynamics of viral communities across varied redox conditions in MBRs. These findings shed light on phage life cycles, and auxiliary genetic capacity such as antibiotic resistance and bacterial quorum sensing within wastewater treatment microbial communities. 

In animal models,Nipbldeficiency phenocopies gene expression changes and birth defects seen in Cornelia de Lange syndrome, the most common cause of which isNipblhaploinsufficiency. Previous studies inNipbl^+/−mice suggested that heart development is abnormal as soon as cardiogenic tissue is formed. To investigate this, we performed single-cell RNA sequencing on wild-type andNipbl^+/−mouse embryos at gastrulation and early cardiac crescent stages.Nipbl^+/−embryos had fewer mesoderm cells than wild-type and altered proportions of mesodermal cell subpopulations. These findings were associated with underexpression of genes implicated in driving specific mesodermal lineages. In addition,Nanogwas found to be overexpressed in all germ layers, and many gene expression changes observed inNipbl^+/−embryos could be attributed toNanogoverexpression. These findings establish a link betweenNipbldeficiency,Nanogoverexpression, and gene expression dysregulation/lineage misallocation, which ultimately manifest as birth defects inNipbl^+/−animals and Cornelia de Lange syndrome.

 

A scalable platform to synthesize ultrathin heavy metals may enable high efficiency charge-to-spin conversion for next-generation spintronics. Here we report the synthesis of air-stable, epitaxially registered monolayer Pb underneath graphene on SiC (0001) by confinement heteroepitaxy (CHet). Diffraction, spectroscopy, and microscopy reveal CHet-based Pb intercalation predominantly exhibits a mottled hexagonal superstructure due to an ordered network of Frenkel-Kontorova-like domain walls. The system’s air stability enables ex-situ spin torque ferromagnetic resonance (ST-FMR) measurements that demonstrate charge-to-spin conversion in graphene/Pb/ferromagnet heterostructures with a 1.5× increase in the effective field ratio compared to control samples. 

Quantum systems have entered a competitive regime in which classical computers must make approximations to represent highly entangled quantum states^1,2. However, in this beyond-classically-exact regime, fidelity comparisons between quantum and classical systems have so far been limited to digital quantum devices^2–5, and it remains unsolved how to estimate the actual entanglement content of experiments⁶. Here, we perform fidelity benchmarking and mixed-state entanglement estimation with a 60-atom analogue Rydberg quantum simulator, reaching a high-entanglement entropy regime in which exact classical simulation becomes impractical. Our benchmarking protocol involves extrapolation from comparisons against an approximate classical algorithm, introduced here, with varying entanglement limits. We then develop and demonstrate an estimator of the experimental mixed-state entanglement⁶, finding our experiment is competitive with state-of-the-art digital quantum devices performing random circuit evolution^2–5. Finally, we compare the experimental fidelity against that achieved by various approximate classical algorithms, and find that only the algorithm we introduce is able to keep pace with the experiment on the classical hardware we use. Our results enable a new model for evaluating the ability of both analogue and digital quantum devices to generate entanglement in the beyond-classically-exact regime, and highlight the evolving divide between quantum and classical systems.

 

We propose a novel collocated projection method for solving the incompressible Navier-Stokes equations with arbitrary boundaries. Our approach employs non-graded octree grids, where all variables are stored at the nodes. To discretize the viscosity and projection steps, we utilize supra-convergent finite difference approximations with sharp boundary treatments. We demonstrate the stability of our projection on uniform grids, identify a sufficient stability condition on adaptive grids, and validate these findings numerically. We further demonstrate the accuracy and capabilities of our solver with several canonical two- and three-dimensional simulations of incompressible fluid flows. Overall, our method is second-order accurate, allows for dynamic grid adaptivity with arbitrary geometries, and reduces the overhead in code development through data collocation. 

Diffuse midline gliomas (DMGs), including diffuse intrinsic pontine glioma, have among the highest mortality rates of all childhood cancers, despite recent advancements in cancer therapeutics. This is partly because, unlike some CNS tumors, the blood-brain barrier (BBB) of DMG tumor vessels remains intact. The BBB prevents the permeation of many molecular therapies into the brain parenchyma, where the cancer cells reside. Focused ultrasound (FUS) with microbubbles has recently emerged as an innovative and exciting technology that non-invasively permeabilizes the BBB in a small focal region with millimeter precision. In this review, current treatment methods and biological barriers to treating DMGs are discussed. State-of-the-art FUS-mediated BBB opening is then examined, with a focus on the effects of various ultrasound parameters and the treatment of DMGs. 

Current optical atomic clocks do not utilize their resources optimally. In particular, an exponential gain in sensitivity could be achieved if multiple atomic ensembles were to be controlled or read out individually, even without entanglement. However, controlling optical transitions locally remains an outstanding challenge for neutral-atom-based clocks and quantum computing platforms. Here we show arbitrary, single-site addressing for an optical transition via sub-wavelength controlled moves of atoms trapped in tweezers. The scheme is highly robust as it relies only on the relative position changes of tweezers and requires no additional addressing beams. Using this technique, we implement single-shot, dual-quadrature readout of Ramsey interferometry using two atomic ensembles simultaneously, and show an enhancement of the usable interrogation time at a given phase-slip error probability. Finally, we program a sequence that performs local dynamical decoupling during Ramsey evolution to evolve three ensembles with variable phase sensitivities, a key ingredient of optimal clock interrogation. Our results demonstrate the potential of fully programmable quantum optical clocks even without entanglement and could be combined with metrologically useful entangled states in the future.