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Free, publicly-accessible full text available February 1, 2025
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Summary Understanding the pronounced seasonal and spatial variation in leaf carboxylation capacity (
V c,max) is critical for determining terrestrial carbon cycling in tropical forests. However, an efficient and scalable approach for predictingV c,maxis still lacking.Here the ability of leaf spectroscopy for rapid estimation of
V c,maxwas tested.V c,maxwas estimated using traditional gas exchange methods, and measured reflectance spectra and leaf age in leaves sampled from tropical forests in Panama and Brazil. These data were used to build a model to predictV c,maxfrom leaf spectra.The results demonstrated that leaf spectroscopy accurately predicts
V c,maxof mature leaves in Panamanian tropical forests (R 2 = 0.90). However, this single‐age model required recalibration when applied to broader leaf demographic classes (i.e. immature leaves). Combined use of spectroscopy models forV c,maxand leaf age enabled construction of theV c,max–age relationship solely from leaf spectra, which agreed with field observations. This suggests that the spectroscopy technique can capture the seasonal variability inV c,max, assuming sufficient sampling across diverse species, leaf ages and canopy environments.This finding will aid development of remote sensing approaches that can be used to characterize
V c,maxin moist tropical forests and enable an efficient means to parameterize and evaluate terrestrial biosphere models. -
Abstract Plant phenology—the timing of cyclic or recurrent biological events in plants—offers insight into the ecology, evolution, and seasonality of plant‐mediated ecosystem processes. Traditionally studied phenologies are readily apparent, such as flowering events, germination timing, and season‐initiating budbreak. However, a broad range of phenologies that are fundamental to the ecology and evolution of plants, and to global biogeochemical cycles and climate change predictions, have been neglected because they are “cryptic”—that is, hidden from view (e.g., root production) or difficult to distinguish and interpret based on common measurements at typical scales of examination (e.g., leaf turnover in evergreen forests). We illustrate how capturing cryptic phenology can advance scientific understanding with two case studies: wood phenology in a deciduous forest of the northeastern USA and leaf phenology in tropical evergreen forests of Amazonia. Drawing on these case studies and other literature, we argue that conceptualizing and characterizing cryptic plant phenology is needed for understanding and accurate prediction at many scales from organisms to ecosystems. We recommend avenues of empirical and modeling research to accelerate discovery of cryptic phenological patterns, to understand their causes and consequences, and to represent these processes in terrestrial biosphere models.