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North America contains abundant Cretaceous continental strata that hosts potential archives of paleoclimate records. While detailed work has been done in Late Cretaceous strata which not only contains archives for stable isotope proxies, but also leaf physiognomic work, less study has occurred in the Early Cretaceous which lack angiosperm-dominated floras. Here we present new paleotemperature estimates from multiple stable isotope proxies including d18O of pedogenic carbonates, clumped isotope paleothermometry, and paired stable isotopes of aquatic and semi-aquatic taxa. The southern-most data originate from the Hensel Formation of South Texas. Clumped isotope paleothermometry of pedogenic carbonates result in temperature estimates between 35°C and 42°C. Clumped isotope paleothermometry of lake and palustrine carbonates from the Cedar Mountain Formation range from 15°C and 31°C for lake carbonates and 20°C to 44°C for palustrine carbonates. Farther north in Wyoming, we utilize the oxygen isotope value of semi aquatic taxa such as crocodilians and turtles to provide a proxy for water and utilize the oxygen isotope value of fish scales and teeth to estimate temperature at 26°C though with significant error. Preliminary clumped isotope paleothermometry provides a temperature of 25°C. The farthest north locality, the Kootenai Formation contains lacustrine, palustrine, and pedogenic carbonates. Utilizing a previous empirically-derived latitudinal gradient of meteoric water, carbonate d18O values were used to estimate a temperature range for the Kootenai between 13°C and 28°C. These values provide a temperature gradient for the Aptian-Albian over the mid latitudes of North America of ~-1°C/degree of latitude. 

Understanding of climate parameters and hydrology during past greenhouse conditions is important to forecasting future climate conditions. Oxygen isotopes of vertebrate bioapatites offer an opportunity to sample conditions across ancient landscapes. 112 samples from a variety of ecological niches were sampled from Cloverly Formation (Lower Cretaceous) micro-vertebrate site V1075 reposited at the Sam Nobel Museum at the University of Oklahoma. These samples were analyzed at the Keck Paleoenvironmental and Environmental Stable Isotope lab at the University of Kansas. Phosphate δ18O values range from between 9‰ to 23‰ vs. SMOW and show significant variability based on interpreted ecological niche of the taxa. Utilizing crocodilian and turtle d18Op values, environmental water oxygen isotope values are interpreted to be approximately -8‰ SMOW. These values are about 2-4‰ enriched relative to past estimates based on latitudinal gradients from penecontemporaneous formations from other latitudes. Based on water isotope estimates, fish δ18O values were used to calculate water temperature values of ~26°C which is consistent with estimated temperatures based on latitudinal gradients for the Cretaceous. In addition, preliminary carbonate clumped isotope paleothermometry suggest similar temperatures. These results along with emerging data from similar age deposits are beginning to build an emerging view of climate on the western side of the Western Interior Seaway during the late Early Cretaceous. 

The Cloverly Formation of Montana and Wyoming preserves abundant nonmarine vertebrate fossils from the mid-Cretaceous, yet its paleoenvironment and faunal niche structure remain poorly understood. We analyzed δ¹⁸Ο_phosphatein over 100 fossil individuals from multiple vertebrate taxa collected from a single microfossil bonebed in Carbon County, Montana.To infer habitat preferences and water-use strategies, we compared δ¹⁸Ο_phosphatevalues within and across taxa. We reconstructed δ¹⁸O_{surface_water}from semi-aquatic reptile values using regressions calibrated with data from modern environments and extant taxa. Using a multi-taxon framework, we estimated warm-season water temperatures from δ¹⁸O_{surface_water}and δ¹⁸Ο_phosphateof lepisosteid (gar) scales, then converted these to air temperatures using a modern climate transfer function. δ¹⁸Ο_phosphatevalues ranged from 9.5‰ to 23.2‰ (VSMOW) and varied across taxa. Aquatic and semi-aquatic groups exhibited lower values than dinosaurian taxa. Our reconstructed mean δ¹⁸O_{surface_water}was −7.9‰ (95% CI: −10.1 to 5.5‰), yielding a warm-season water temperature of 26°C and an air temperature of 24°C. Intertaxon differences reflect niche partitioning and suggest primary isotopic signals are preserved. Unexpectedly high values in Bernissartiid-like neosuchian teeth may indicate greater ecohydrological diversity than previously recognized. Our δ¹⁸O_{surface_water}estimate aligns with other Aptian-Albian proxies but exceeds model-based predictions, likely due to outdated assumptions underlying the model. The MAWSAT estimate falls within the upper range of model-data assimilation outputs. These results provide new context for ecological structure in the Cloverly fauna and offer the first quantitative temperature estimate for the Formation, helping to define baseline conditions between the Aptian-Albian Cold Snap and the Cretaceous Thermal Maximum. 

Quasi-brittle fracture mechanics is used to evaluate fracture of human cortical bone in aging. The approach is demonstrated using cortical bone bars extracted from one 92-year-old human male cadaver. In-situ fracture mechanics experiments in a 3D X-ray microscope are conducted. The evolution of the fracture process zone is documented. Fully developed fracture process zone lengths at peak load are found to span about three osteon diameters. Crack deflection and arrest at cement lines is a key process to build extrinsic toughness. Strength and toughness are found as size-dependent, not only for laboratory-scale experimental specimens but also for the whole femur. A scaling law for the length fracture process zone is used. Then, size-independent, tissue fracture properties are calculated. Linear elastic fracture mechanics applied to laboratory beam specimens underestimates the tissue toughness by 60%. Tissue fracture properties are used to predict the load capacity of the femur in bending within the range of documented data. The quasi-brittle fracture mechanics approach allows for the assessment of the combined effect of bone quantity and bone quality on fracture risk. However, further work is needed considering a larger range of subjects and in the model validation at the organ length scale. 

Three extended abstracts are part of this special publication of Anatomical Record of the 14th Symposium on Mesozoic Terrestrial Ecosystems and Biota 

3D Printing (3DP), also known as Additive Manufacturing (AM) is the latest production technology. Its popularity in fabricating functional parts in all fields is growing day by day. The range of 3D printed products is limitless, including glass frames to hearing aids. It is thus important to train educators and students regarding this cutting-edge technology so that they become familiar with the functionality and implementation of it in several courses, laboratories, and projects. This paper reports several novel developments which have been implemented in the past few years, including details of these unique practices and feedback received from the educators and students.