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Creators/Authors contains: "Allen, Mark G."

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  1. Abstract

    Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity, as well as rapid or real-time operation. Furthermore, microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation. Herein, we present microfabricated needle-shaped microwell impedance sensors for rapid-sample-to-answer, label-free detection of cytokines, and other biomarkers. The microneedle form factor allows sensors to be utilized in transcutaneous or transvascular sensing applications. In vitro, experimental characterization confirmed sensor specificity and sensitivity to multiple proteins of interest. Mechanical characterization demonstrated sufficient microneedle robustness for transcutaneous insertion, as well as preserved sensor function postinsertion. We further utilized these sensors to carry out real-time in vivo quantification of human interleukin 8 (hIL8) concentration levels in the blood of transgenic mice that endogenously express hIL8. To assess sensor functionality, hIL8 concentration levels in serum samples from the same mice were quantified by ELISA. Excellent agreement between real-time in vivo sensor readings in blood and subsequent ELISA serum assays was observed over multiple transgenic mice expressing hIL8 concentrations from 62 pg/mL to 539 ng/mL.

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  2. Abstract

    We study the ionization and excitation structure of the interstellar medium in the late-stage gas-rich galaxy merger NGC 6240 using a suite of emission-line maps at ∼25 pc resolution from the Hubble Space Telescope, Keck/NIRC2 with Adaptive Optics, and the Atacama Large Millimeter/submillimeter Array (ALMA). NGC 6240 hosts a superwind driven by intense star formation and/or one or both of two active nuclei; the outflows produce bubbles and filaments seen in shock tracers from warm molecular gas (H22.12μm) to optical ionized gas ([Oiii], [Nii], [Sii], and [Oi]) and hot plasma (FeXXV). In the most distinct bubble, we see a clear shock front traced by high [Oiii]/Hβand [Oiii]/[Oi]. Cool molecular gas (CO(2−1)) is only present near the base of the bubble, toward the nuclei launching the outflow. We interpret the lack of molecular gas outside the bubble to mean that the shock front is not responsible for dissociating molecular gas, and conclude that the molecular clouds are partly shielded and either entrained briefly in the outflow, or left undisturbed while the hot wind flows around them. Elsewhere in the galaxy, shock-excited H2extends at least ∼4 kpc from the nuclei, tracing molecular gas even warmer than that between the nuclei, where the two galaxies’ interstellar media are colliding. A ridgeline of high [Oiii]/Hβemission along the eastern arm aligns with the southern nucleus’ stellar disk minor axis; optical integral field spectroscopy from WiFeS suggests this highly ionized gas is centered at systemic velocity and likely photoionized by direct line of sight to the southern active galactic nucleus.

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