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Ultra-high-molecular-weight polyethylene (UHMWPE) components for orthopedic implants have historically been integrated into metal backings by direct-compression molding (DCM). However, metal backings are costly, stiffer than cortical bone, and may be associated with medical imaging distortion and metal release. Hybrid-manufactured DCM UHMWPE overmolded additively manufactured polyetheretherketone (PEEK) structural components could offer an alternative solution, but are yet to be explored. In this study, five different porous topologies (grid, triangular, honeycomb, octahedral, and gyroid) and three surface feature sizes (low, medium, and high) were implemented into the top surface of digital cylindrical specimens prior to being 3D printed in PEEK and then overmolded with UHMWPE. Separation forces were recorded as 1.97–3.86 kN, therefore matching and bettering the historical industry values (2–3 kN) recorded for DCM UHMWPE metal components. Infill topology affected failure mechanism (Type 1 or 2) and obtained separation forces, with shapes having greater sidewall numbers (honeycomb-60%) and interconnectivity (gyroid-30%) through their builds, tolerating higher transmitted forces. Surface feature size also had an impact on applied load, whereby those with low infill-%s generally recorded lower levels of performance vs. medium and high infill strategies. These preliminary findings suggest that hybrid-manufactured structural composites could replace metal backings and produce orthopedic implants with high-performing polymer–polymer interfaces. 

In garnets from eclogites and blueschists formed within the subduction setting, fine-scale, oscillatory elemental zoning is a common feature, sometimes considered to record open-system fluid exchange during prograde metamorphism. We present oxygen isotope data for garnets with such zoning from five exhumed subduction zone complexes. Short length scale fluctuations in elemental and oxygen isotope zoning (which are themselves spatially decoupled) cannot be linked to open-system fluid exchange during garnet crystallization in all samples; these data do not provide evidence for a genetic relationship between elemental oscillations and fluid fluxing. However, garnets from one setting do provide clear evidence for syn-growth ingress of elementally and isotopically buffering fluids, a process that operated simultaneously with the formation of elemental oscillations. Our findings indicate multiple mechanisms of chemical transfer operate at the grain–rock scale during subduction, and that some subduction zone rocks may experience only limited interaction with external prograde fluids. These results are consistent with a picture of highly heterogenous volatile transfer during subduction, and suggest that some proportion of the fluid inventory inherited at shallow depths may be transferred to sub-arc depths. 

Abstract We study the ionization and excitation structure of the interstellar medium in the late-stage gas-rich galaxy merger NGC 6240 using a suite of emission-line maps at ∼25 pc resolution from the Hubble Space Telescope, Keck/NIRC2 with Adaptive Optics, and the Atacama Large Millimeter/submillimeter Array (ALMA). NGC 6240 hosts a superwind driven by intense star formation and/or one or both of two active nuclei; the outflows produce bubbles and filaments seen in shock tracers from warm molecular gas (H₂2.12μm) to optical ionized gas ([Oiii], [Nii], [Sii], and [Oi]) and hot plasma (FeXXV). In the most distinct bubble, we see a clear shock front traced by high [Oiii]/Hβand [Oiii]/[Oi]. Cool molecular gas (CO(2−1)) is only present near the base of the bubble, toward the nuclei launching the outflow. We interpret the lack of molecular gas outside the bubble to mean that the shock front is not responsible for dissociating molecular gas, and conclude that the molecular clouds are partly shielded and either entrained briefly in the outflow, or left undisturbed while the hot wind flows around them. Elsewhere in the galaxy, shock-excited H₂extends at least ∼4 kpc from the nuclei, tracing molecular gas even warmer than that between the nuclei, where the two galaxies’ interstellar media are colliding. A ridgeline of high [Oiii]/Hβemission along the eastern arm aligns with the southern nucleus’ stellar disk minor axis; optical integral field spectroscopy from WiFeS suggests this highly ionized gas is centered at systemic velocity and likely photoionized by direct line of sight to the southern active galactic nucleus. 

Plastics pollution represents a global environmental crisis. In response, microbes are evolving the capacity to utilize synthetic polymers as carbon and energy sources. Recently, Ideonella sakaiensis was reported to secrete a two-enzyme system to deconstruct polyethylene terephthalate (PET) to its constituent monomers. Specifically, the I. sakaiensis PETase depolymerizes PET, liberating soluble products, including mono(2-hydroxyethyl) terephthalate (MHET), which is cleaved to terephthalic acid and ethylene glycol by MHETase. Here, we report a 1.6 Å resolution MHETase structure, illustrating that the MHETase core domain is similar to PETase, capped by a lid domain. Simulations of the catalytic itinerary predict that MHETase follows the canonical two-step serine hydrolase mechanism. Bioinformatics analysis suggests that MHETase evolved from ferulic acid esterases, and two homologous enzymes are shown to exhibit MHET turnover. Analysis of the two homologous enzymes and the MHETase S131G mutant demonstrates the importance of this residue for accommodation of MHET in the active site. We also demonstrate that the MHETase lid is crucial for hydrolysis of MHET and, furthermore, that MHETase does not turnover mono(2-hydroxyethyl)-furanoate or mono(2-hydroxyethyl)-isophthalate. A highly synergistic relationship between PETase and MHETase was observed for the conversion of amorphous PET film to monomers across all nonzero MHETase concentrations tested. Finally, we compare the performance of MHETase:PETase chimeric proteins of varying linker lengths, which all exhibit improved PET and MHET turnover relative to the free enzymes. Together, these results offer insights into the two-enzyme PET depolymerization system and will inform future efforts in the biological deconstruction and upcycling of mixed plastics. 

Abstract Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.