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The isolation ofBradyrhizobiumstrain I71 expands the distribution of acetylene-consuming microbes to include a group of economically important microorganisms. Members ofBradyrhizobiumare well studied for their abilities to improve plant health and increase crop yields by providing bioavailable nitrogen.

A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93^T, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93^Twas capable of acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 and in the presence of 10–45 g l⁻¹NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed that SFB93^Trepresented a member of the genusSyntrophotaleawith highest 16S rRNA gene sequence similarities toSyntrophotalea acetylenicaDSM 3246^T(96.6 %),Syntrophotalea carbinolicaDSM 2380^T(96.5 %), andSyntrophotalea venetianaDSM 2394^T(96.7 %). Genome sequencing revealed a genome size of 3.22 Mbp and a DNA G+C content of 53.4 %. SFB93^Thad low genome-wide average nucleotide identity (81–87.5 %) and <70 % digital DNA–DNA hybridization value with other members of the genusSyntrophotalea. The phylogenetic position of SFB93^Twithin the familySyntrophotaleaceaeand as a novel member of the genusSyntrophotaleawas confirmed via phylogenetic reconstruction based on concatenated alignments of 92 bacterial core genes. On the basis of the results of phenotypic, genotypic and phylogenetic analyses, a novel species,Syntrophotalea acetylenivoranssp. nov., is proposed, with SFB93^T(=DSM 106009^T=JCM 33327^T=ATCC TSD-118^T) as the type strain.