Search for: All records

This study evaluates the beneficial effects of discharging nanosecond pulse transient plasma (NPTP) in a coaxial electrostatic precipitator for capturing nanoscale soot particles (∼50 nm) produced by an ethylene flame. 

The incorporation of the nonstandard amino acid para-nitro-L-phenylalanine (pN-Phe) within proteins has been used for diverse applications, including the termination of immune self-tolerance. However, the requirement for the provision of chemically synthesized pN-Phe to cells limits the contexts where this technology can be harnessed. Here we report the construction of a live bacterial producer of synthetic nitrated proteins by coupling metabolic engineering and genetic code expansion. We achieved the biosynthesis of pN-Phe in Escherichia coli by creating a pathway that features a previously uncharacterized nonheme diiron N-monooxygenase, which resulted in pN-Phe titers of 820 ± 130 µM after optimization. After we identified an orthogonal translation system that exhibited selectivity toward pN-Phe rather than a precursor metabolite, we constructed a single strain that incorporated biosynthesized pN-Phe within a specific site of a reporter protein. Overall, our study has created a foundational technology platform for distributed and autonomous production of nitrated proteins. 

Abstract The cosmic merger history of supermassive black hole binaries (SMBHBs) is expected to produce a low-frequency gravitational wave background (GWB). Here we investigate how signs of the discrete nature of this GWB can manifest in pulsar timing arrays (PTAs) through excursions from, and breaks in, the expected $f_{\mathrm{GW}}^{-2/3}$power law of the GWB strain spectrum. To do this, we create a semianalytic SMBHB population model, fit to North American Nanohertz Observatory for Gravitational Waves (NANOGrav’s) 15 yr GWB amplitude, and with 1000 realizations, we study the populations’ characteristic strain and residual spectra. Comparing our models to the NANOGrav 15 yr spectrum, we find two interesting excursions from the power law. The first, at 2 nHz, is below our GWB realizations with ap-value significancep= 0.05–0.06 (≈1.8σ–1.9σ). The second, at 16 nHz, is above our GWB realizations withp= 0.04–0.15 (≈1.4σ–2.1σ). We explore the properties of a loud SMBHB that could cause such an excursion. Our simulations also show that the expected number of SMBHBs decreases by 3 orders of magnitude, from ∼10⁶to ∼10³, between 2 and 20 nHz. This causes a break in the strain spectrum as the stochasticity of the background breaks down at ${26}_{-19}^{+28}\mathrm{nHz}$, consistent with predictions pre-dating GWB measurements. The diminished GWB signal from SMBHBs at frequencies above the 26 nHz break opens a window for PTAs to detect continuous GWs from individual SMBHBs or GWs from the early Universe.