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Creators/Authors contains: "Capponi, Sara"

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  1. Seaweeds, particularly the red seaweed Asparagopsis taxiformis, produce and sequester bromomethanes, which are known for mitigating methane emissions in ruminants when used as a feed supplement. Bromomethane synthesis requires hydrogen peroxide (H2O2). We developed a staining assay utilizing 3,3′-diaminobenzidine (DAB) for identifying H2O2 in three groups of seaweeds (red, brown, and green), including intensely pigmented species. Our findings indicate the previously identified "gland cell" in Asparagopsis taxiformis, responsible for bromoform synthesis and retention, is a specialized large organelle rich in H2O2. Our study introduces an effective survey tool to identify promising seaweed species abundant in bromoform from diverse marine habitats. 
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  2. ABSTRACT Biophysics is an interdisciplinary pursuit requiring researchers with knowledge and skills in several areas. Optical instruments and computers are fundamental tools in biophysics research to collect and analyze data. We developed a 1-semester Optical Engineering Laboratory course to teach image processing, optical engineering, and research skills to undergraduate students majoring in biology and biochemistry. With the use of development systems on students' laptops and in the cloud, students learned image processing with Python and OpenCV. Each student constructed a microprocessor-based lensless holographic microscope, gaining hands-on experience with optical engineering. The class culminated in original, student-designed research projects. All lectures, hands-on labs, and student research projects were performed both in person and remotely, in response to the COVID-19 pandemic. 
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