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Kim, Taejin ; Sathyanarayana, Sandesh Dhawaskar ; Chen, Siqi ; Im, Youngbin ; Zhang, Xiaoxi ; Ha, Sangtae ; Joe-Wong, Carlee ( , IEEE Journal on Selected Areas in Communications)
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Chen, Siqi ; Chang, Yong-Kui ( , IMA Journal of Mathematical Control and Information)
Abstract This paper is mainly concerned with a controlled multi-term fractional evolution equation in Banach spaces. Firstly, we give formula of its mild solutions and show the existence result for the problem via $\omega $-sectorial operator technique. Secondly, we establish the Lagrange optimal control and time optimal control for the system invoked by the nonlocal Cauchy problems of multi-term fractional evolution equation by properties of resolvent operators.
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Kim, Taejin ; Sathyanarayana, Sandesh Dhawaskar ; Chen, Siqi ; Im, Youngbin ; Zhang, Xiaoxi ; Ha, Sangtae ; Joe-Wong, Carlee ( , IEEE INFOCOM 2022 - IEEE Conference on Computer Communications)
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Zou, Roger S. ; Gavrilov, Momcilo ; Liu, Yang ; Rasoloson, Dominique ; Conte, Madison ; Hardick, Justin ; Shen, Leo ; Chen, Siqi ; Pekosz, Andrew ; Seydoux, Geraldine ; et al ( , Communications Biology)
Abstract Nucleic acid detection is essential for numerous biomedical applications, but often requires complex protocols and/or suffers false-positive readouts. Here, we describe SENTINEL, an approach that combines isothermal amplification with a sequence-specific degradation method to detect nucleic acids with high sensitivity and sequence-specificity. Target single-stranded RNA or double-stranded DNA molecules are amplified by loop-mediated isothermal amplification (LAMP) and subsequently degraded by the combined action of lambda exonuclease and a sequence-specific DNA endonuclease (e.g., Cas9). By combining the sensitivity of LAMP with the precision of DNA endonucleases, the protocol achieves attomolar limits of detection while differentiating between sequences that differ by only one or two base pairs. The protocol requires less than an hour to complete using a 65 °C heat block and fluorometer, and detects SARS-CoV-2 virus particles in human saliva and nasopharyngeal swabs with high sensitivity.