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Membrane viscosity is an important property of cell biology, which determines cellular function, development and disease progression. Various experimental and computational methods have been developed to investigate the mechanics of cells. However, there have been no experimental measurements of the membrane viscosity at high-frequencies in live cells. High frequency measurements are important because they can probe viscoelastic effects. Here, we investigate the membrane viscosity at gigahertz-frequencies through the damping of the acoustic vibrations of gold nanoplates. The experiments are modeled using a continuum mechanics theory which reveals that the membranes display viscoelasticity, with an estimated relaxation time of ca. ps. We further demonstrate that membrane viscoelasticity can be used to differentiate a cancerous cell line (the human glioblastoma cells LN-18) from a normal cell line (the mouse brain microvascular endothelial cells bEnd.3). The viscosity of cancerous cells LN-18 is lower than that of healthy cells bEnd.3 by a factor of three. The results indicate promising applications of characterizing membrane viscoelasticity at gigahertz-frequency in cell diagnosis. 

Chatter in thin-walled parts is easy to occur in the process of machining, so the analysis of the stability of thin-walled parts has always been a research hotspot. In this paper, considering the influence of cutter eccentricity on milling force first, the coefficients of milling force were able to be identified by combining the milling force model with genetic algorithm. The results show that this method can obtain the milling force coefficients only by one experiment, and the accuracy is higher. Then the tool point Frequency Response Function (FRF) for a given combination can be calculated by using the Receptance coupling substructure analysis (RCSA) method that uses Timoshenko beam theory. Finally, the milling system can be divided into three types by aspect ratio. That is, when aspect ratio is less than 0.03, the system is considered to be a rigid tool-flexible workpiece system, but aspect ratio is between 0.03 and 0.2, the system is considered to be a flexible tool-flexible system, then aspect ratio is greater than 0.2, the system is considered to be a flexible cutter-rigid workpiece system.