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Conventional strategies for biological sensing require complex workflows to capture, isolate, and prepare biomolecules for specific and high-sensitivity detection. Instruments that facilitate these processes are usually cumbersome, reducing user-friendliness and accessibility. Here, we present a multichannel acoustic separator with biospecific and acoustically responsive microparticles to simplify workflows and shorten the time needed to isolate and detect biomarkers from complex fluids. The multichannel acoustic separator is 3D-printed and supports 12 acoustofluidic trapping channels that isolate the biospecific particles from off-target contaminants in the fluid. Fluid flow through the channels is driven by a semi-continuous siphon, which eliminates the need for fluid pumps. We tested the system for purifying three disparate biomolecules in individual and multiplexed formats, as well as the purification of IgA from whole blood for detection in approximately 70 min. 

Abstract Induced‐charge electroosmosis (ICEO) offers a practical approach to drive microscale flows by application of AC electric fields across polarizable surfaces, enabling diverse functions including microfluidic pumping, active cargo transport, and biosensing. While ICEO along pristine surfaces is well‐understood, practical applications of ICEO often require surface modifications that affect ICEO flows in a manner that is poorly understood. Here, this study introduces dielectrophoretic (DEP) polarizability measurement, DPM, as a method to study effects of surface modifications on surface polarizability and ICEO flows. The method entails DEP trapping of probe particles and analysis of their equilibrium motions to measure polarizability. This DPM‐generated polarizability data is then used to predict effects of surface modifications on ICEO flows and reveal the contribution of additional factors affecting ICEO. It compares predictions with experimentally observed changes to the speed of Janus particles traveling by ICEO‐driven induced‐charge electrophoresis. This study shows that DPM enables prediction of decreased particle speed upon protein capture by functional Janus particles and reveals that increased speed of polymer‐modified Janus particles likely arises from hydrodynamic factors. Overall, this work lays the foundation for investigating new ICEO‐driven systems with applications in complex environments, potentially including those encountered in biosensing, remediation, or cargo delivery. 

Most biosensing techniques require complex processing steps that generate prolonged workflows and introduce potential points of error. Here, we report an acoustic pipette to purify and label biomarkers in 70 minutes. A key aspect of this technology is the use of functional negative acoustic contrast particles (fNACPs), which display biorecognition motifs for the specific capture of biomarkers from whole blood. Because of their large size and compressibility, the fNACPs robustly trap along the pressure antinodes of a standing wave and separate from blood components in under 60 seconds with >99% efficiency. fNACPs are subsequently fluorescently labeled in the pipette and are analyzed by both a custom, portable fluorimeter and flow cytometer. We demonstrate the detection of anti-ovalbumin antibodies from blood at picomolar levels (35 to 60 pM) with integrated controls showing minimal nonspecific adsorption. Overall, this system offers a simple and versatile approach for the rapid, sensitive, and specific capture of biomolecules. 

We demonstrate the feasibility of probing the charged lepton-flavor-violating decay mu+ -> e+ X0 for the presence of a slow-moving neutral boson X0 capable of undergoing gravitational binding to large structures and, as such, able to participate in some cosmological scenarios. A short exposure to surface antimuons from beam line M20 at TRIUMF generates a branching ratio limit of <1E-5. This is comparable to or better than previous searches for this channel, although in a thus- far-unexplored region of X0 phase space very close to the kinematic limit of the decay, where m_X0 approaches m_mu+ . The future improved sensitivity of the method using a customized p-type point-contact germanium detector is described. 

Active particles, or micromotors, locally dissipate energy to drive locomotion at small length scales. The type of trajectory is generally fixed and dictated by the geometry and composition of the particle, which can be challenging to tune using conventional fabrication procedures. Here, we report a simple, bottom-up method to magnetically assemble gold-coated polystyrene Janus particles into “locked” clusters that display diverse trajectories when stimulated by AC electric fields. The orientation of particles within each cluster gives rise to distinct modes of locomotion, including translational, rotational, trochoidal, helical, and orbital. We model this system using a simplified rigid beads model and demonstrate qualitative agreement between the predicted and experimentally observed cluster trajectories. Overall, this system provides a facile means to scalably create micromotors with a range of well-defined motions from discrete building blocks. 

PICO bubble chambers have exceptional sensitivity to inelastic dark matter-nucleus interactions due to a combination of their extended nuclear recoil energy detection window from a few keV to O(100 keV) or more and the use of iodine as a heavy target. Inelastic dark matter-nucleus scattering is interesting for studying the properties of dark matter, where many theoretical scenarios have been developed. This study reports the results of a search for dark matter inelastic scattering with the PICO-60 bubble chambers. The analysis reported here comprises physics runs from PICO-60 bubble chambers using CF3I and C3F8. The CF3I run consisted of 36.8 kg of CF3I reaching an exposure of 3415 kg-day operating at thermodynamic thresholds between 7 and 20 keV. The C3F8 runs consisted of 52 kg of C3F8 reaching exposures of 1404 kg-day and 1167 kg-day running at thermodynamic thresholds of 2.45 keV and 3.29 keV, respectively. The analysis disfavors various scenarios, in a wide region of parameter space, that provide a feasible explanation of the signal observed by DAMA, assuming an inelastic interaction, considering that the PICO CF3I bubble chamber used iodine as the target material.