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Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perceptionAbstract KAI2 proteins are plant α/β hydrolase receptors which perceive smoke-derived butenolide signals and endogenous, yet unidentified KAI2-ligands (KLs). The number of functional KAI2 receptors varies among species and KAI2 gene duplication and sub-functionalization likely plays an adaptative role by altering specificity towards different KLs. Legumes represent one of the largest families of flowering plants and contain many agronomic crops. Prior to their diversification, KAI2 underwent duplication resulting in KAI2A and KAI2B. Here we demonstrate that Pisum sativum KAI2A and KAI2B are active receptors and enzymes with divergent ligand stereoselectivity. KAI2B has a higher affinity for and hydrolyses a broader range of substrates including strigolactone-like stereoisomers. We determine the crystal structures of PsKAI2B in apo and butenolide-bound states. The biochemical, structural, and mass spectra analyses of KAI2s reveal a transient intermediate on the catalytic serine and a stable adduct on the catalytic histidine, confirming its role as a bona fide enzyme. Our work uncovers the stereoselectivity of ligand perception and catalysis by diverged KAI2 receptors and proposes adaptive sensitivity to KAR/KL and strigolactones by KAI2B.Free, publicly-accessible full text available December 1, 2023
Multi-scale analysis of the Monoceros OB 1 star-forming region: II. Colliding filaments in the Monoceros OB1 molecular cloudContext. We started a multi-scale analysis of star formation in G202.3+2.5, an intertwined filamentary sub-region of the Monoceros OB1 molecular complex, in order to provide observational constraints on current theories and models that attempt to explain star formation globally. In the first paper (Paper I), we examined the distributions of dense cores and protostars and found enhanced star formation activity in the junction region of the filaments. Aims. In this second paper, we aim to unveil the connections between the core and filament evolutions, and between the filament dynamics and the global evolution of the cloud. Methods. We characterise the gas dynamics and energy balance in different parts of G202.3+2.5 using infrared observations from the Herschel and WISE telescopes and molecular tracers observed with the IRAM 30-m and TRAO 14-m telescopes. The velocity field of the cloud is examined and velocity-coherent structures are identified, characterised, and put in perspective with the cloud environment. Results. Two main velocity components are revealed, well separated in radial velocities in the north and merged around the location of intense N 2 H + emission in the centre of G202.3+2.5 where Paper I found the peak of star formation activity. We show that the relativemore »